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  • Featured Product
  • KD/KO Validated

CoraLite® Plus 647-conjugated S100A11 Polyclonal antibody

S100A11 Polyclonal Antibody for FC (Intra)

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

FC (Intra)

Conjugate

CoraLite® Plus 647 Fluorescent Dye

Cat no : CL647-10237

Synonyms

Protein S100-A11, Protein S100 C, Protein S100 A11, MLN70, MLN 70

Validation Data Gallery

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  • Flow cytometry (FC) experiment of MCF-7 cells using CoraLite® Plus 647-conjugated S100A11 Polyclonal a (CL647-10237)

    FC experiment of MCF-7 using CL647-10237

    1X10^6 MCF-7 cells were intracellularly stained with 0.2 ug CoraLite® Plus 647 Anti-Human S100A11 (CL647-10237) (red), or 0.2 ug isotype control (blue). Cells were fixed and permeabilized with Transcription Factor Staining Buffer Kit (PF00011).

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Tested Applications

Positive FC (Intra) detected inMCF-7 cells

Recommended dilution

ApplicationDilution
Flow Cytometry (FC) (INTRA)FC (INTRA) : 0.20 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

CL647-10237 targets S100A11 in FC (Intra) applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen S100A11 fusion protein Ag0343 相同性解析による交差性が予測される生物種
Full Name S100 calcium binding protein A11
Calculated molecular weight 105 aa, 12 kDa
Observed molecular weight 12 kDa
GenBank accession numberBC001410
Gene symbol S100A11
Gene ID (NCBI) 6282
Conjugate CoraLite® Plus 647 Fluorescent Dye
Excitation/Emission maxima wavelengths654 nm / 674 nm
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
Storage ConditionsStore at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

S100A11 (also known as S100C or calziggarin), is a member of the S100 family of proteins containing 2 EF-hand calcium-binding motifs (PMID: 18694925). S100 proteins are localized in the cytoplasm and/or nucleus of a wide range of cells, and involved in the regulation of a number of cellular processes such as cell cycle progression and differentiation. First discovered in 1989, S100A11 has since been proposed to have direct biological functions in an assortment of physiological processes such as endo- and exocytosis, regulation of enzyme activity, cell growth and regulation, apoptosis and inflammation (PMID: 15241500). Chromosomal rearrangements and altered expression of S100A11 have been implicated in tumor metastasis. This S100A11 antibody (10237-1-AP) has also been instrumental in investigations into S100A11's role in the development of brain tumors in tuberous sclerosis complex (TSC) patients (PMID: 20133820).

Protocols

Product Specific Protocols
FC protocol for CL Plus 647 S100A11 antibody CL647-10237Download protocol
Standard Protocols
Click here to view our Standard Protocols