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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

FSP1/S100A4 Recombinant antibody, PBS Only (Detector)

FSP1/S100A4 Recombinant Antibody for WB, IF/ICC, FC (Intra), Cytometric bead array, Indirect ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IF/ICC, FC (Intra), Cytometric bead array, Indirect ELISA

Conjugate

Unconjugated

CloneNo.

241448A12

Cat no : 84221-1-PBS

Synonyms

S100A4, Fibroblast-specific protein-1, Fibroblast-specific protein 1, Fibroblast Specific Protein, CAPL

Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using FSP1/S100A4 Recombinant antibody (84221-1-RR)

    WB analysis using 84221-1-RR (same clone as 84221-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 84221-1-RR (S100A4 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 84221-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of HeLa cells using FSP1/S100A4 Recombinant antibody (84221-1-RR)

    IF Staining of HeLa using 84221-1-RR (same clone as 84221-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed HeLa cells using S100A4 antibody (84221-1-RR, Clone: 241448A12 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-Phalloidin (red). This data was developed using the same antibody clone with 84221-1-PBS in a different storage buffer formulation.

  • Flow cytometry (FC) experiment of HeLa cells using FSP1/S100A4 Recombinant antibody (84221-1-RR)

    FC experiment of HeLa using 84221-1-RR (same clone as 84221-1-PBS)

    1x10^6 HeLa cells were intracellularly stained with 0.25 ug S100A4 Recombinant antibody (84221-1-RR, Clone:241448A12) and APC-Conjugated Goat Anti-Rabbit IgG(H+L)(red), or 0.25 ug Isotype Control (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C). This data was developed using the same antibody clone with 84221-1-PBS in a different storage buffer formulation.

  • Flow cytometry (FC) experiment of Jurkat cells using FSP1/S100A4 Recombinant antibody (84221-1-RR)

    FC experiment of Jurkat using 84221-1-RR (same clone as 84221-1-PBS)

    1x10^6 Jurkat cells were intracellularly stained with 0.25 ug S100A4 Recombinant antibody (84221-1-RR, Clone:241448A12) and APC-Conjugated Goat Anti-Rabbit IgG(H+L)(red), or 0.25 ug Isotype Control (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C). This data was developed using the same antibody clone with 84221-1-PBS in a different storage buffer formulation.

  • Cytometric bead array standard curve of MP01155-2

    Cytometric bead array standard curve of MP01155-2

    Cytometric bead array standard curve of MP01155-2, S100A4 Recombinant Matched Antibody Pair, PBS Only. Capture antibody: 84221-2-PBS. Detection antibody: 84221-1-PBS. Standard: Ag9019. Range: 0.625-80 ng/mL.

  • Affinity and Kinetic Characterization of 84221-1-RR

    Affinity and Kinetic Characterization of 84221-1-RR

    Biolayer interferometry (BLl) kinetic assays of 84221-1-RR against Human S100A4 were performed. The affinity constant is 2.75 nM.

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Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

84221-1-PBS targets FSP1/S100A4 as part of a matched antibody pair:

MP01155-2: 84221-2-PBS capture and 84221-1-PBS detection (validated in Cytometric bead array)

Unconjugated rabbit recombinant monoclonal antibody in PBS only (BSA and azide free) storage buffer at a concentration of 1 mg/mL, ready for conjugation. Created using Proteintech’s proprietary in-house recombinant technology. Recombinant production enables unrivalled batch-to-batch consistency, easy scale-up, and future security of supply.

This conjugation ready format makes antibodies ideal for use in many applications including: ELISAs, multiplex assays requiring matched pairs, mass cytometry, and multiplex imaging applications.Antibody use should be optimized by the end user for each application and assay.

Tested Reactivity human
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Immunogen FSP1/S100A4 fusion protein Ag9019 相同性解析による交差性が予測される生物種
Full Name S100 calcium binding protein A4
Calculated molecular weight 101 aa, 12 kDa
Observed molecular weight 10-12 kDa
GenBank accession numberBC016300
Gene symbol S100A4
Gene ID (NCBI) 6275
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.

Background Information

S100A4 is a member of the S100 family of calcium-binding proteins. The S100 family members have been involved in the regulation of a number of cellular processes such as cell cycle progression and differentiation. S100A4 is known to localize to and function in the nucleus, cytoplasm of cells, and the extracellular space. S100A4 has also been shown to be associated with tumor growth, motility, invasion, metastasis, angiogenesis, apoptosis, and chemoresistance. It is a fibroblast-specific protein associated with mesenchymal cell morphology and motility, is expressed during epithelial-mesenchymal transformations (EMT) in vivo (PMID: 9362334). It is a specific prognostic marker for renal survival in patients with IgAN (PMID: 16105038). It is also an improved marker for lung fibroblasts that could be useful for investigating the pathogenesis of pulmonary fibrosis(PMID: 15618458). Overexpression of S100A4 is correlated with a worse prognosis inpatients with various types of cancer.