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Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using S100A4 Recombinant antibody (84221-1-RR)

    WB analysis using 84221-1-RR

    Various lysates were subjected to SDS PAGE followed by western blot with 84221-1-RR (S100A4 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

  • Immunofluorescence (IF) / fluorescent staining of HeLa cells using S100A4 Recombinant antibody (84221-1-RR)

    IF Staining of HeLa using 84221-1-RR

    Immunofluorescent analysis of (4% PFA) fixed HeLa cells using S100A4 antibody (84221-1-RR, Clone: 241448A12 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-Phalloidin (red).

  • Flow cytometry (FC) experiment of HeLa cells using S100A4 Recombinant antibody (84221-1-RR)

    FC experiment of HeLa using 84221-1-RR

    1x10^6 HeLa cells were intracellularly stained with 0.25 ug S100A4 Recombinant antibody (84221-1-RR, Clone:241448A12) and APC-Conjugated Goat Anti-Rabbit IgG(H+L)(red), or 0.25 ug Isotype Control (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

  • Flow cytometry (FC) experiment of Jurkat cells using S100A4 Recombinant antibody (84221-1-RR)

    FC experiment of Jurkat using 84221-1-RR

    1x10^6 Jurkat cells were intracellularly stained with 0.25 ug S100A4 Recombinant antibody (84221-1-RR, Clone:241448A12) and APC-Conjugated Goat Anti-Rabbit IgG(H+L)(red), or 0.25 ug Isotype Control (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

  • Affinity and Kinetic Characterization of 84221-1-RR

    Affinity and Kinetic Characterization of 84221-1-RR

    Biolayer interferometry (BLl) kinetic assays of 84221-1-RR against Human S100A4 were performed. The affinity constant is 2.75 nM.

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S100A4 Recombinant antibody

S100A4 Uni-rAbTM Recombinant Antibody for WB, IF/ICC, FC (Intra), ELISA
Cat No. 84221-1-RR
Clone No.241448A12

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IF/ICC, FC (Intra), ELISA

Synonyms

Fibroblast-specific protein-1, Fibroblast-specific protein 1, Fibroblast Specific Protein, CAPL, Calvasculin

Formulation:  PBS and Azide
PBS and Azide
PBS Only
Conjugate:  Unconjugated
Unconjugated
Size/Concentration: 

-/ -

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テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Positive WB detected inA549 cells, HeLa cells, NIH/3T3 cells, C6 cells
Positive IF/ICC detected inHeLa cells
Positive FC (Intra) detected inJurkat cells, HeLa cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:5000-1:50000
Immunofluorescence (IF)/ICCIF/ICC : 1:200-1:800
Flow Cytometry (FC) (INTRA)FC (INTRA) : 0.25 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

84221-1-RR targets S100A4 in WB, IF/ICC, FC (Intra), ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Immunogen S100A4 fusion protein Ag9019 相同性解析による交差性が予測される生物種
Full Name S100 calcium binding protein A4
Calculated molecular weight 101 aa, 12 kDa
Observed molecular weight 10-12 kDa
GenBank accession numberBC016300
Gene Symbol S100A4
Gene ID (NCBI) 6275
ENSEMBL Gene IDENSG00000196154
RRIDAB_3671775
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
UNIPROT IDP26447
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

S100A4 is a member of the S100 family of calcium-binding proteins. The S100 family members have been involved in the regulation of a number of cellular processes such as cell cycle progression and differentiation. S100A4 is known to localize to and function in the nucleus, cytoplasm of cells, and the extracellular space. S100A4 has also been shown to be associated with tumor growth, motility, invasion, metastasis, angiogenesis, apoptosis, and chemoresistance. It is a fibroblast-specific protein associated with mesenchymal cell morphology and motility, is expressed during epithelial-mesenchymal transformations (EMT) in vivo (PMID: 9362334). It is a specific prognostic marker for renal survival in patients with IgAN (PMID: 16105038). It is also an improved marker for lung fibroblasts that could be useful for investigating the pathogenesis of pulmonary fibrosis(PMID: 15618458). Overexpression of S100A4 is correlated with a worse prognosis inpatients with various types of cancer.

Protocols

Product Specific Protocols
WB protocol for S100A4 antibody 84221-1-RRDownload protocol
IF protocol for S100A4 antibody 84221-1-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols