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S100A6 Monoclonal antibody

S100A6 Monoclonal Antibody for WB, IHC, IF/ICC, ELISA
Cat No. 66098-1-Ig
Clone No.3D11C8

Host / Isotype

Mouse / IgG1

Reactivity

human

Applications

WB, IHC, IF/ICC, ELISA

MLN 4, Growth factor-inducible protein 2A9, Calcyclin, CACY, CABP

Formulation:  PBS and Azide
PBS and Azide
PBS Only
Conjugate:  Unconjugated
Unconjugated
CoraLite® Plus 488
Size/Concentration: 

-/ -


ご購入について

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国内在庫・納期について

約2万点のプロテインテック製品をコスモバイオ社物流センター(国内)に在庫しています。国内在庫の有無はコスモバイオ社ホームページの「品番検索」でカタログ番号を検索して確認できます。


保証・サポートについて

テクニカルサポートまたはご購入後1年間の交換/補填対応を承ります。詳細はこちらをご覧ください。


Tested Applications

Positive WB detected inHeLa cells, A549 cells
Positive IHC detected inhuman colon cancer tissue, human liver cancer tissue, human pancreas cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inHepG2 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2000
Immunohistochemistry (IHC)IHC : 1:50-1:500
Immunofluorescence (IF)/ICCIF/ICC : 1:200-1:800
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Published Applications

WBSee 1 publications below

Product Information

66098-1-Ig targets S100A6 in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Cited Reactivityhuman
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Immunogen S100A6 fusion protein Ag17606 相同性解析による交差性が予測される生物種
Full Name S100 calcium binding protein A6
Calculated molecular weight 10 kDa
Observed molecular weight 10 kDa
GenBank accession numberBC001431
Gene Symbol S100A6
Gene ID (NCBI) 6277
RRIDAB_2881497
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
UNIPROT IDP06703
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

S100A6 is also named as calcyclin, prolactin receptor-associated protein (PRA), growth factor-inducible protein 2A9 ir MLN4. It belongs to S100 family of low molecular weight, acidic, calcium-binding proteins which contain two EF-hand calcium binding sites. S100A6 may function as a calcium sensor to activate several processes in the calcium signal transduction pathway of cell growth, proliferation, secretion and exocytosis. Although S100A6 is a cytoplasmic protein, it can be located in the nuclear and cytoplasmic membranes in the presence of Ca2+ (PMID: 37670392).

Protocols

Product Specific Protocols
WB protocol for S100A6 antibody 66098-1-IgDownload protocol
IHC protocol for S100A6 antibody 66098-1-IgDownload protocol
IF protocol for S100A6 antibody 66098-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanWB

Open Med (Wars)

S100A6 promotes proliferation and migration of HepG2 cells via increased ubiquitin-dependent degradation of p53.

Authors - Dongqiang Song
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