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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

S100A6 Monoclonal antibody, PBS Only

S100A6 Monoclonal Antibody for WB, IHC, IF/ICC, Indirect ELISA

Host / Isotype

Mouse / IgG1

Reactivity

human

Applications

WB, IHC, IF/ICC, Indirect ELISA

Conjugate

Unconjugated

CloneNo.

3D11C8

Cat no : 66098-1-PBS

Synonyms

MLN 4, Growth factor-inducible protein 2A9, Calcyclin, CACY, CABP

Validation Data Gallery

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  • Western Blot (WB) analysis of HeLa cells using S100A6 Monoclonal antibody (66098-1-Ig)

    WB analysis of HeLa using 66098-1-Ig (same clone as 66098-1-PBS)

    HeLa cells were subjected to SDS PAGE followed by western blot with 66098-1-Ig (S100A6 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66098-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of A549 cells using S100A6 Monoclonal antibody (66098-1-Ig)

    WB analysis of A549 using 66098-1-Ig (same clone as 66098-1-PBS)

    A549 cells were subjected to SDS PAGE followed by western blot with 66098-1-Ig (S100A6 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66098-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human colon cancer tissue using S100A6 Monoclonal antibody (66098-1-Ig)

    IHC staining of human colon cancer using 66098-1-Ig (same clone as 66098-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 66098-1-Ig (S100A6 antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66098-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human colon cancer tissue using S100A6 Monoclonal antibody (66098-1-Ig)

    IHC staining of human colon cancer using 66098-1-Ig (same clone as 66098-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 66098-1-Ig (S100A6 antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66098-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human pancreas cancer tissue using S100A6 Monoclonal antibody (66098-1-Ig)

    IHC staining of human pancreas cancer using 66098-1-Ig (same clone as 66098-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human pancreas cancer tissue slide using 66098-1-Ig (S100A6 antibody) at dilution of 1:2000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66098-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human pancreas cancer tissue using S100A6 Monoclonal antibody (66098-1-Ig)

    IHC staining of human pancreas cancer using 66098-1-Ig (same clone as 66098-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human pancreas cancer tissue slide using 66098-1-Ig (S100A6 antibody) at dilution of 1:2000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66098-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human colon cancer tissue using S100A6 Monoclonal antibody (66098-1-Ig)

    IHC staining of human colon cancer using 66098-1-Ig (same clone as 66098-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human colon cancer slide using 66098-1-Ig (S100A6 Antibody) at dilution of 1:50. This data was developed using the same antibody clone with 66098-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human liver cancer tissue using S100A6 Monoclonal antibody (66098-1-Ig)

    IHC staining of human liver cancer using 66098-1-Ig (same clone as 66098-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human liver cancer slide using 66098-1-Ig (S100A6 Antibody) at dilution of 1:50. This data was developed using the same antibody clone with 66098-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human liver cancer tissue using S100A6 Monoclonal antibody (66098-1-Ig)

    IHC staining of human liver cancer using 66098-1-Ig (same clone as 66098-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human liver cancer slide using 66098-1-Ig (S100A6 Antibody) at dilution of 1:50. This data was developed using the same antibody clone with 66098-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human colon cancer tissue using S100A6 Monoclonal antibody (66098-1-Ig)

    IHC staining of human colon cancer using 66098-1-Ig (same clone as 66098-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human colon cancer slide using 66098-1-Ig (S100A6 Antibody) at dilution of 1:50. This data was developed using the same antibody clone with 66098-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of HepG2 cells using S100A6 Monoclonal antibody (66098-1-Ig)

    IF Staining of HepG2 using 66098-1-Ig (same clone as 66098-1-PBS)

    Immunofluorescent analysis of (-20°C Ethanol) fixed HepG2 cells using S100A6 antibody (66098-1-Ig, Clone: 3D11C8 ) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 66098-1-PBS in a different storage buffer formulation.

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Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

66098-1-PBS targets S100A6 in WB, IHC, IF/ICC, Indirect ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Immunogen S100A6 fusion protein Ag17606 相同性解析による交差性が予測される生物種
Full Name S100 calcium binding protein A6
Calculated molecular weight 10 kDa
Observed molecular weight 10 kDa
GenBank accession numberBC001431
Gene symbol S100A6
Gene ID (NCBI) 6277
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.

Background Information

S100A6 is also named as calcyclin, prolactin receptor-associated protein (PRA), growth factor-inducible protein 2A9 ir MLN4. It belongs to S100 family of low molecular weight, acidic, calcium-binding proteins which contain two EF-hand calcium binding sites. S100A6 may function as a calcium sensor to activate several processes in the calcium signal transduction pathway of cell growth, proliferation, secretion and exocytosis. Although S100A6 is a cytoplasmic protein, it can be located in the nuclear and cytoplasmic membranes in the presence of Ca2+ (PMID: 37670392).