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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

S100A9 Recombinant antibody, PBS Only (Detector)

S100A9 Recombinant Antibody for IF/ICC, FC (Intra), Cytometric bead array, Indirect ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

IF/ICC, FC (Intra), Cytometric bead array, Indirect ELISA

Conjugate

Unconjugated

CloneNo.

240539F4

Cat no : 83578-6-PBS

Synonyms

CFAG, Calprotectin L1H subunit, Calgranulin-B, Calgranulin B, CAGB

Validation Data Gallery

Filter:
  • Immunofluorescence (IF) / fluorescent staining of RAW 264.7 cells using S100A9 Recombinant antibody (83578-6-RR)

    IF Staining of RAW 264.7 using 83578-6-RR (same clone as 83578-6-PBS)

    Immunofluorescent analysis of (4% PFA) fixed RAW 264.7 cells using S100A9 antibody (83578-6-RR, Clone: 240539F4 ) at dilution of 1:250 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) (SA00013-2). This data was developed using the same antibody clone with 83578-6-PBS in a different storage buffer formulation.

  • Flow cytometry (FC) experiment of A431 cells using S100A9 Recombinant antibody, PBS Only (Detector) (83578-6-PBS)

    FC experiment of A431 using 83578-6-PBS

    1x10^6 A431 cells were intracellularly stained with 0.25 ug S100A9 Recombinant antibody, PBS Only (Detector) (83578-6-PBS, Clone:240539F4) and APC-Conjugated Goat Anti-Rabbit IgG(H+L)(red), or 0.25 ug Isotype Control (blue). Cells were fixed and permeabilized with True-Nuclear Transcription Factor Buffer Set.

  • Flow cytometry (FC) experiment of A431 cells using S100A9 Recombinant antibody (83578-6-RR)

    FC experiment of A431 using 83578-6-RR (same clone as 83578-6-PBS)

    1x10^6 A431 cells were intracellularly stained with 0.25 ug S100A9 Recombinant antibody (83578-6-RR, Clone:240539F4) and APC-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L)(red), or 0.25 ug Isotype Control (blue). Cells were fixed and permeabilized with Transcription Factor Staining Buffer Kit (PF00011). This data was developed using the same antibody clone with 83578-6-PBS in a different storage buffer formulation.

  • Flow cytometry (FC) experiment of Jurkat cells using S100A9 Recombinant antibody (83578-6-RR)

    FC experiment of Jurkat using 83578-6-RR (same clone as 83578-6-PBS)

    1x10^6 Jurkat cells were intracellularly stained with 0.25 ug S100A9 Recombinant antibody (83578-6-RR, Clone:240539F4) and APC-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L)(red), or 0.25 ug Isotype Control (blue). Cells were fixed and permeabilized with Transcription Factor Staining Buffer Kit (PF00011). This data was developed using the same antibody clone with 83578-6-PBS in a different storage buffer formulation.

  • Cytometric bead array standard curve of MP00569-3

    Cytometric bead array standard curve of MP00569-3

    Cytometric bead array standard curve of MP00569-3, S100A9 Recombinant Matched Antibody Pair, PBS Only. Capture antibody: 83578-2-PBS. Detection antibody: 83578-6-PBS. Standard: Ag25764. Range: 0.625-40 ng/mL.

  • Affinity and Kinetic Characterization of 83578-6-RR

    Affinity and Kinetic Characterization of 83578-6-RR

    Biolayer interferometry (BLl) kinetic assays of 83578-6-RR against Human S100A9 were performed. The affinity constant is 1.19 nM.

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Tested Applications

Positive FC (Intra) detected inA431 cells

Recommended dilution

ApplicationDilution
Flow Cytometry (FC) (INTRA)FC (INTRA) : 0.25 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

83578-6-PBS targets S100A9 as part of a matched antibody pair:

MP00569-3: 83578-2-PBS capture and 83578-6-PBS detection (validated in Cytometric bead array)

Unconjugated rabbit recombinant monoclonal antibody in PBS only (BSA and azide free) storage buffer at a concentration of 1 mg/mL, ready for conjugation. Created using Proteintech’s proprietary in-house recombinant technology. Recombinant production enables unrivalled batch-to-batch consistency, easy scale-up, and future security of supply.

This conjugation ready format makes antibodies ideal for use in many applications including: ELISAs, multiplex assays requiring matched pairs, mass cytometry, and multiplex imaging applications.Antibody use should be optimized by the end user for each application and assay.

Tested Reactivity human
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Immunogen S100A9 fusion protein Ag25764 相同性解析による交差性が予測される生物種
Full Name S100 calcium binding protein A9
Calculated molecular weight 13 kDa
GenBank accession numberBC047681
Gene symbol S100A9
Gene ID (NCBI) 6280
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.

Background Information

S100A9 is a calcium binding protein as a member of the S100 family of proteins. S100 proteins are low molecular weight (9 to 14 kDa) intracellular calcium-binding proteins that control key cellular pathways including regulation of the cytoskeleton, cell migration and adhesion, and host oxidative defense. S100A9 may exist as a homodimer, heterodimer (24 kDa) with an S100A8 partner (S100A8/A9), or as a heterotetramer (28 kDa) with an S100A8 partner(S100A8/A9). S100A8 and S100A9 are found intracellularly in granulocytes, monocytes, and early differentiation stages of macrophages.