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Validation Data Gallery

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  • Immunofluorescence (IF) / fluorescent staining of RAW 264.7 cells using S100A9 Recombinant antibody (83578-6-RR)

    IF Staining of RAW 264.7 using 83578-6-RR

    Immunofluorescent analysis of (4% PFA) fixed RAW 264.7 cells using S100A9 antibody (83578-6-RR, Clone: 240539F4 ) at dilution of 1:250 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2).

  • Flow cytometry (FC) experiment of A431 cells using S100A9 Recombinant antibody (83578-6-RR)

    FC experiment of A431 using 83578-6-RR

    1x10^6 A431 cells were intracellularly stained with 0.25 ug S100A9 Recombinant antibody (83578-6-RR, Clone:240539F4) and APC-Conjugated Goat Anti-Rabbit IgG(H+L)(red), or 0.25 ug Isotype Control (blue). Cells were fixed and permeabilized with Transcription Factor Staining Buffer Kit (PF00011).

  • Flow cytometry (FC) experiment of Jurkat cells using S100A9 Recombinant antibody (83578-6-RR)

    FC experiment of Jurkat using 83578-6-RR

    1x10^6 Jurkat cells were intracellularly stained with 0.25 ug S100A9 Recombinant antibody (83578-6-RR, Clone:240539F4) and APC-Conjugated Goat Anti-Rabbit IgG(H+L)(red), or 0.25 ug Isotype Control (blue). Cells were fixed and permeabilized with Transcription Factor Staining Buffer Kit (PF00011).

  • Affinity and Kinetic Characterization of 83578-6-RR

    Affinity and Kinetic Characterization of 83578-6-RR

    Biolayer interferometry (BLl) kinetic assays of 83578-6-RR against Human S100A9 were performed. The affinity constant is 1.19 nM.

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S100A9 Recombinant antibody

S100A9 Uni-rAbTM Recombinant Antibody for IF/ICC, FC (Intra), ELISA
Cat No. 83578-6-RR
Clone No.240539F4

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

IF/ICC, FC (Intra), ELISA

Synonyms

CFAG, Calprotectin L1H subunit, Calgranulin-B, Calgranulin B, CAGB

Formulation:  PBS and Azide
PBS and Azide
PBS Only
Conjugate:  Unconjugated
Unconjugated
Size/Concentration: 

-/ -

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テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Positive IF/ICC detected inRAW 264.7 cells
Positive FC (Intra) detected inA431 cells, Jurkat cells

Recommended dilution

ApplicationDilution
Immunofluorescence (IF)/ICCIF/ICC : 1:125-1:500
Flow Cytometry (FC) (INTRA)FC (INTRA) : 0.25 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

83578-6-RR targets S100A9 in IF/ICC, FC (Intra), ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Immunogen S100A9 fusion protein Ag25764 相同性解析による交差性が予測される生物種
Full Name S100 calcium binding protein A9
Calculated molecular weight 13 kDa
GenBank accession numberBC047681
Gene Symbol S100A9
Gene ID (NCBI) 6280
RRIDAB_3671191
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
UNIPROT IDP06702
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

S100A9 is a calcium binding protein as a member of the S100 family of proteins. S100 proteins are low molecular weight (9 to 14 kDa) intracellular calcium-binding proteins that control key cellular pathways including regulation of the cytoskeleton, cell migration and adhesion, and host oxidative defense. S100A9 may exist as a homodimer, heterodimer (24 kDa) with an S100A8 partner (S100A8/A9), or as a heterotetramer (28 kDa) with an S100A8 partner(S100A8/A9). S100A8 and S100A9 are found intracellularly in granulocytes, monocytes, and early differentiation stages of macrophages.

Protocols

Product Specific Protocols
IF protocol for S100A9 antibody 83578-6-RRDownload protocol
FC protocol for S100A9 antibody 83578-6-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols