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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

S6 Ribosomal protein Recombinant antibody, PBS Only

S6 Ribosomal protein Recombinant Antibody for WB, IF/ICC, Indirect ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat, zebrafish

Applications

WB, IF/ICC, Indirect ELISA

Conjugate

Unconjugated

CloneNo.

6L9

Cat no : 80208-1-PBS

Synonyms

RPS6, S6, RP S6, ribosomal protein S6, Phosphoprotein NP33

Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using S6 Ribosomal protein Recombinant antibody (80208-1-RR)

    WB analysis using 80208-1-RR (same clone as 80208-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 80208-1-RR (S6 Ribosomal protein antibody) at dilution of 1:15000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 80208-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of various lysates using S6 Ribosomal protein Recombinant antibody (80208-1-RR)

    WB analysis using 80208-1-RR (same clone as 80208-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 80208-1-RR (S6 Ribosomal protein antibody) at dilution of 1:15000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 80208-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of zebrafish tissue using S6 Ribosomal protein Recombinant antibody (80208-1-RR)

    WB analysis of zebrafish using 80208-1-RR (same clone as 80208-1-PBS)

    zebrafish tissue were subjected to SDS PAGE followed by western blot with 80208-1-RR (S6 Ribosomal protein antibody) at dilution of 1:15000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 80208-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of HepG2 cells using S6 Ribosomal protein Recombinant antibody (80208-1-RR)

    IF Staining of HepG2 using 80208-1-RR (same clone as 80208-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using S6 Ribosomal protein antibody (80208-1-RR, Clone: 6L9 ) at dilution of 1:250 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L). This data was developed using the same antibody clone with 80208-1-PBS in a different storage buffer formulation.

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Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

80208-1-PBS targets S6 Ribosomal protein in WB, IF/ICC, Indirect ELISA applications and shows reactivity with human, mouse, rat, zebrafish samples.

Tested Reactivity human, mouse, rat, zebrafish
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Immunogen Peptide 相同性解析による交差性が予測される生物種
Full Name ribosomal protein S6
Calculated molecular weight 29 kDa
Observed molecular weight 32 kDa
GenBank accession numberBC000524
Gene symbol RPS6
Gene ID (NCBI) 6194
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.

Background Information

Ribosomal protein S6 (RPS6),Phosphoprotein NP33.It may play an important role in controlling cell growth and proliferation through the selective translation of particular classes of mRNA.Ribosomal protein S6 is the major substrate of protein kinases in eukaryote ribosomes. The phosphorylation is stimulated by growth factors, tumor promoting agents, and mitogens. It is dephosphorylated at growth arrest. Phosphorylated at Ser-235 and Ser-236 by RPS6KA1 and RPS6KA3; phosphorylation at these sites facilitates the assembly of the preinitiation complex.