S6 Ribosomal protein Recombinant antibody

S6 Ribosomal protein Recombinant Antibody for WB, IF/ICC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat, zebrafish

Applications

WB, IF/ICC, ELISA

Conjugate

Unconjugated

CloneNo.

6L9

Cat no : 80208-1-RR

Synonyms

RPS6, S6, RP S6, ribosomal protein S6, Phosphoprotein NP33


☆対象製品が30%オフ!組換えタンパク質&免染用試薬 >>> キャンペーン実施中!


Tested Applications

Positive WB detected inLNCaP cells, RAW 264.7 cells, zebrafish tissue, HeLa cells, HEK-293 cells, HepG2 cells, Jurkat cells, HSC-T6 cells, 4T1 cells
Positive IF/ICC detected inHepG2 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:5000-1:50000
Immunofluorescence (IF)/ICCIF/ICC : 1:125-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Published Applications

WBSee 3 publications below

Product Information

80208-1-RR targets S6 Ribosomal protein in WB, IF/ICC, ELISA applications and shows reactivity with human, mouse, rat, zebrafish samples.

Tested Reactivity human, mouse, rat, zebrafish
Cited Reactivityhuman, mouse
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Immunogen Peptide 相同性解析による交差性が予測される生物種
Full Name ribosomal protein S6
Calculated molecular weight 29 kDa
Observed molecular weight 32 kDa
GenBank accession numberBC000524
Gene symbol RPS6
Gene ID (NCBI) 6194
RRIDAB_2918876
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

Ribosomal protein S6 (RPS6),Phosphoprotein NP33.It may play an important role in controlling cell growth and proliferation through the selective translation of particular classes of mRNA.Ribosomal protein S6 is the major substrate of protein kinases in eukaryote ribosomes. The phosphorylation is stimulated by growth factors, tumor promoting agents, and mitogens. It is dephosphorylated at growth arrest. Phosphorylated at Ser-235 and Ser-236 by RPS6KA1 and RPS6KA3; phosphorylation at these sites facilitates the assembly of the preinitiation complex.

Protocols

Product Specific Protocols
WB protocol for S6 Ribosomal protein antibody 80208-1-RRDownload protocol
IF protocol for S6 Ribosomal protein antibody 80208-1-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
mouseWB

ACS Omega

ROS-responsive MSC-derived Exosome Mimetics Carrying MHY1485 Alleviate Renal Ischemia Reperfusion Injury through Multiple Mechanisms

Authors - Zhiyu Qian
humanWB

J Biol Chem

TFE3-SLC36A1 axis promotes resistance to glucose starvation in kidney cancer cells

Authors - Suli Lv
humanWB

Biomark Res

Deciphering metabolic heterogeneity in retinoblastoma unravels the role of monocarboxylate transporter 1 in tumor progression

Authors - Junjie Tang