S6 Ribosomal protein Recombinant antibody
S6 Ribosomal protein Recombinant Antibody for WB, IF/ICC, ELISA
Host / Isotype
Rabbit / IgG
Reactivity
human, mouse, rat, zebrafish
Applications
WB, IF/ICC, ELISA
Conjugate
Unconjugated
CloneNo.
6L9
Cat no : 80208-1-RR
Synonyms
Validation Data Gallery
Tested Applications
| Positive WB detected in | LNCaP cells, RAW 264.7 cells, zebrafish tissue, HeLa cells, HEK-293 cells, HepG2 cells, Jurkat cells, HSC-T6 cells, 4T1 cells |
| Positive IF/ICC detected in | HepG2 cells |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:5000-1:50000 |
| Immunofluorescence (IF)/ICC | IF/ICC : 1:125-1:500 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Published Applications
| WB | See 3 publications below |
Product Information
80208-1-RR targets S6 Ribosomal protein in WB, IF/ICC, ELISA applications and shows reactivity with human, mouse, rat, zebrafish samples.
| Tested Reactivity | human, mouse, rat, zebrafish |
| Cited Reactivity | human, mouse |
| Host / Isotype | Rabbit / IgG |
| Class | Recombinant |
| Type | Antibody |
| Immunogen | Peptide 相同性解析による交差性が予測される生物種 |
| Full Name | ribosomal protein S6 |
| Calculated molecular weight | 29 kDa |
| Observed molecular weight | 32 kDa |
| GenBank accession number | BC000524 |
| Gene symbol | RPS6 |
| Gene ID (NCBI) | 6194 |
| RRID | AB_2918876 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Protein A purification |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
Background Information
Ribosomal protein S6 (RPS6),Phosphoprotein NP33.It may play an important role in controlling cell growth and proliferation through the selective translation of particular classes of mRNA.Ribosomal protein S6 is the major substrate of protein kinases in eukaryote ribosomes. The phosphorylation is stimulated by growth factors, tumor promoting agents, and mitogens. It is dephosphorylated at growth arrest. Phosphorylated at Ser-235 and Ser-236 by RPS6KA1 and RPS6KA3; phosphorylation at these sites facilitates the assembly of the preinitiation complex.
Protocols
| Product Specific Protocols | |
|---|---|
| WB protocol for S6 Ribosomal protein antibody 80208-1-RR | Download protocol |
| IF protocol for S6 Ribosomal protein antibody 80208-1-RR | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
| Species | Application | Title |
|---|---|---|
J Biol Chem TFE3-SLC36A1 axis promotes resistance to glucose starvation in kidney cancer cells | ||
ACS Omega ROS-responsive MSC-derived Exosome Mimetics Carrying MHY1485 Alleviate Renal Ischemia Reperfusion Injury through Multiple Mechanisms | ||
Biomark Res Deciphering metabolic heterogeneity in retinoblastoma unravels the role of monocarboxylate transporter 1 in tumor progression |
