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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

CoraLite® Plus 488-conjugated S6 Ribosomal protein Recombinant antibody

S6 Ribosomal protein Recombinant Antibody for IF/ICC

Host / Isotype

Rabbit / IgG

Reactivity

Human, rat, mouse, zabrafish

Applications

IF/ICC

Conjugate

CoraLite® Plus 488 Fluorescent Dye

CloneNo.

6L9

Cat no : CL488-80208

Synonyms

40S ribosomal protein S6, Phosphoprotein NP33, ribosomal protein S6, RPS6

Validation Data Gallery

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  • Immunofluorescence (IF) / fluorescent staining of HepG2 cells using CoraLite® Plus 488-conjugated S6 Ribosomal protein (CL488-80208)

    IF Staining of HepG2 using CL488-80208

    Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using CoraLite® Plus 488 RPS6 antibody (CL488-80208, Clone: 6L9 ) at dilution of 1:200, CL594-Phalloidin (red).

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Tested Applications

Positive IF/ICC detected inHepG2 cells

Recommended dilution

ApplicationDilution
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

CL488-80208 targets S6 Ribosomal protein in IF/ICC applications and shows reactivity with Human, rat, mouse, zabrafish samples.

Tested Reactivity Human, rat, mouse, zabrafish
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Immunogen Peptide 相同性解析による交差性が予測される生物種
Full Name ribosomal protein S6
Calculated molecular weight 29 kDa
Observed molecular weight 32 kDa
GenBank accession numberBC000524
Gene symbol RPS6
Gene ID (NCBI) 6194
Conjugate CoraLite® Plus 488 Fluorescent Dye
Excitation/Emission maxima wavelengths493 nm / 522 nm
Form Liquid
Purification MethodProtein A purification
Storage Buffer PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
Storage ConditionsStore at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

Ribosomal protein S6 (RPS6),Phosphoprotein NP33.It may play an important role in controlling cell growth and proliferation through the selective translation of particular classes of mRNA.Ribosomal protein S6 is the major substrate of protein kinases in eukaryote ribosomes. The phosphorylation is stimulated by growth factors, tumor promoting agents, and mitogens. It is dephosphorylated at growth arrest. Phosphorylated at Ser-235 and Ser-236 by RPS6KA1 and RPS6KA3; phosphorylation at these sites facilitates the assembly of the preinitiation complex.

Protocols

Product Specific Protocols
IF protocol for CL Plus 488 S6 Ribosomal protein antibody CL488-80208Download protocol
Standard Protocols
Click here to view our Standard Protocols