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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

CoraLite® Plus 488-conjugated SAFB2 Polyclonal antibody

SAFB2 Polyclonal Antibody for IF/ICC, FC (Intra)

Host / Isotype

Rabbit / IgG

Reactivity

human, rat

Applications

IF/ICC, FC (Intra)

Conjugate

CoraLite® Plus 488 Fluorescent Dye

Cat no : CL488-11642

Synonyms

SAF-B2, SAF B2, KIAA0138

Validation Data Gallery

Filter:
  • Immunofluorescence (IF) / fluorescent staining of U2OS cells using CoraLite® Plus 488-conjugated SAFB2 Polyclonal ant (CL488-11642)

    IF Staining of U2OS using CL488-11642

    Immunofluorescent analysis of (4% PFA) fixed U2OS cells using CoraLite® Plus 488 SAFB2 antibody (CL488-11642) at dilution of 1:200, CL594-Phalloidin (red).

  • Flow cytometry (FC) experiment of U2OS cells using CoraLite® Plus 488-conjugated SAFB2 Polyclonal ant (CL488-11642)

    FC experiment of U2OS using CL488-11642

    1x10^6 U2OS cells were intracellularly stained with 0.4 ug Coralite® Plus 488 Safb2 Polyclonal Antibody (CL488-11642)(red), or 0.4 ug CoraLite® Plus 488-conjugated Rabbit IgG control Rabbit PolyAb (CL488-30000) (blue). Cells were fixed and permeabilized with Transcription Factor Staining Buffer Kit (PF00011).

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Tested Applications

Positive IF/ICC detected inU2OS cells
Positive FC (Intra) detected inU2OS cells

Recommended dilution

ApplicationDilution
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
Flow Cytometry (FC) (INTRA)FC (INTRA) : 0.40 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

CL488-11642 targets SAFB2 in IF/ICC, FC (Intra) applications and shows reactivity with human, rat samples.

Tested Reactivity human, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen SAFB2 fusion protein Ag2230 相同性解析による交差性が予測される生物種
Full Name scaffold attachment factor B2
Calculated molecular weight528aa,57 kDa; 953aa,107 kDa
Observed molecular weight 130 kDa
GenBank accession numberBC025279
Gene symbol SAFB2
Gene ID (NCBI) 9667
Conjugate CoraLite® Plus 488 Fluorescent Dye
Excitation/Emission maxima wavelengths493 nm / 522 nm
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
Storage ConditionsStore at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

SAFB2, also named as Scaffold attachment factor B2 or KIAA0138, is a 953 amino acid protein, which contains one SAP domain and one RRM (RNA recognition motif) domain. SAFB2 expressed at high levels in the CNS and at low levels in the liver. It also is expressed in a wide number of breast cancer cell lines. SAFB2 binds to scaffold/matrix attachment region (S/MAR) DNA and can function as an estrogen receptor corepressor and can also inhibit cell proliferation.

Protocols

Product Specific Protocols
IF protocol for CL Plus 488 SAFB2 antibody CL488-11642Download protocol
FC protocol for CL Plus 488 SAFB2 antibody CL488-11642Download protocol
Standard Protocols
Click here to view our Standard Protocols