Validation Data Gallery
Tested Applications
| Positive WB detected in | K-562 cells |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:500-1:2000 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Published Applications
| WB | See 2 publications below |
| IHC | See 1 publications below |
Product Information
66236-1-Ig targets SALL4 in WB, IHC, ELISA applications and shows reactivity with human samples.
| Tested Reactivity | human |
| Cited Reactivity | human |
| Host / Isotype | Mouse / IgG1 |
| Class | Monoclonal |
| Type | Antibody |
| Immunogen | SALL4 fusion protein Ag17480 相同性解析による交差性が予測される生物種 |
| Full Name | sal-like 4 (Drosophila) |
| Calculated molecular weight | 1053 aa, 112 kDa |
| Observed molecular weight | 165 kDa |
| GenBank accession number | BC111714 |
| Gene Symbol | SALL4 |
| Gene ID (NCBI) | 57167 |
| RRID | AB_2881625 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Protein G purification |
| UNIPROT ID | Q9UJQ4 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
Background Information
SALL4, also named as Sal-like protein 4 or Zinc finger protein 797, Contains 7 C2H2-type zinc fingers and belongs to the sal C2H2-type zinc-finger protein family. SALL4 is constitutively expressed in acute myeloid leukemia. The constitutive expression of SALL4in mice is sufficient to induce MDS-like symptoms and transformation to AML that is transplantable. SALL4 is able to bind beta-catenin and activate the Wnt/beta-catenin signaling pathway. Sequence analysis of the larger cDNA fragment isolated revealed a single, large open-reading frame, designated as SALL4A, that started from a strong consensus initiation sequence and was expected to encode 1053 amino acids. The other splicing variant of SALL4, designated SALL4B, lacked the region corresponding to amino acids 385 to 820 of the full-length SALL4A. The putative protein encoded by SALL4B cDNA was expected to consist of 617 amino acids.
Protocols
| Product Specific Protocols | |
|---|---|
| WB protocol for SALL4 antibody 66236-1-Ig | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
| Species | Application | Title |
|---|---|---|
Discov Oncol HOXC12 promotes the invasion and migration of gastric cancer cells by upregulating SALL4 and activating Wnt/β-catenin signaling pathway | ||
Cancer Chemother Pharmacol Sorafenib combined with dasatinib therapy inhibits cell viability, migration, and angiogenesis synergistically in hepatocellular carcinoma. | ||