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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

SALL4 Monoclonal antibody, PBS Only

SALL4 Monoclonal Antibody for WB, Indirect ELISA

Host / Isotype

Mouse / IgG1

Reactivity

human

Applications

WB, Indirect ELISA

Conjugate

Unconjugated

CloneNo.

2B5B2

Cat no : 66236-1-PBS

Synonyms

DRRS, HSAL4, sal like 4 (Drosophila), Sal like protein 4, SALL4, Zinc finger protein SALL4, ZNF797

Validation Data Gallery

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  • Western Blot (WB) analysis of K-562 cells using SALL4 Monoclonal antibody (66236-1-Ig)

    WB analysis of K-562 using 66236-1-Ig (same clone as 66236-1-PBS)

    K-562 cells were subjected to SDS PAGE followed by western blot with 66236-1-Ig (SALL4 Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66236-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of K-562 cells using SALL4 Monoclonal antibody (66236-1-Ig)

    WB analysis of K-562 using 66236-1-Ig (same clone as 66236-1-PBS)

    K-562 cells were subjected to SDS PAGE followed by western blot with 66236-1-Ig (SALL4 Antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66236-1-PBS in a different storage buffer formulation.

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Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

66236-1-PBS targets SALL4 in WB, Indirect ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Immunogen SALL4 fusion protein Ag17480 相同性解析による交差性が予測される生物種
Full Name sal-like 4 (Drosophila)
Calculated molecular weight 1053 aa, 112 kDa
Observed molecular weight 165 kDa
GenBank accession numberBC111714
Gene symbol SALL4
Gene ID (NCBI) 57167
Conjugate Unconjugated
Form Liquid
Purification MethodProtein G purification
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.

Background Information

SALL4, also named as Sal-like protein 4 or Zinc finger protein 797, Contains 7 C2H2-type zinc fingers and belongs to the sal C2H2-type zinc-finger protein family. SALL4 is constitutively expressed in acute myeloid leukemia. The constitutive expression of SALL4in mice is sufficient to induce MDS-like symptoms and transformation to AML that is transplantable. SALL4 is able to bind beta-catenin and activate the Wnt/beta-catenin signaling pathway. Sequence analysis of the larger cDNA fragment isolated revealed a single, large open-reading frame, designated as SALL4A, that started from a strong consensus initiation sequence and was expected to encode 1053 amino acids. The other splicing variant of SALL4, designated SALL4B, lacked the region corresponding to amino acids 385 to 820 of the full-length SALL4A. The putative protein encoded by SALL4B cDNA was expected to consist of 617 amino acids.