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  • Featured Product
  • KD/KO Validated

CoraLite® Plus 488-conjugated SALL4 Polyclonal antibody

SALL4 Polyclonal Antibody for IF/ICC

Host / Isotype

Rabbit / IgG

Reactivity

human, rat

Applications

IF/ICC

Conjugate

CoraLite® Plus 488 Fluorescent Dye

Cat no : CL488-24500

Synonyms

dJ1112F19.1, DRRS, HSAL4, sal like 4 (Drosophila), Sal like protein 4, SALL4, Zinc finger protein SALL4, ZNF797

Validation Data Gallery

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  • Immunofluorescence (IF) / fluorescent staining of Caco-2 cells using CoraLite® Plus 488-conjugated SALL4 Polyclonal ant (CL488-24500)

    IF Staining of Caco-2 using CL488-24500

    Immunofluorescent analysis of (4% PFA) fixed Caco-2 cells using CoraLite® Plus 488 SALL4 antibody (CL488-24500) at dilution of 1:200.

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Tested Applications

Positive IF/ICC detected inCaco-2 cells

Recommended dilution

ApplicationDilution
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

CL488-24500 targets SALL4 in IF/ICC applications and shows reactivity with human, rat samples.

Tested Reactivity human, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen SALL4 fusion protein Ag17480 相同性解析による交差性が予測される生物種
Full Name sal-like 4 (Drosophila)
Calculated molecular weight 1053 aa, 112 kDa
Observed molecular weight 66-75 kDa
GenBank accession numberBC111714
Gene symbol SALL4
Gene ID (NCBI) 57167
Conjugate CoraLite® Plus 488 Fluorescent Dye
Excitation/Emission maxima wavelengths493 nm / 522 nm
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
Storage ConditionsStore at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

SALL4, also named Sal-like protein 4 or Zinc finger protein 797, Contains 7 C2H2-type zinc fingers and belongs to the sal C2H2-type zinc-finger protein family. SALL4 is constitutively expressed in acute myeloid leukemia. The constitutive expression of SALL4 in mice is sufficient to induce MDS-like symptoms and transformation to AML that is transplantable. SALL4 is able to bind beta-catenin and activate the Wnt/beta-catenin signaling pathway. Sequence analysis of the larger cDNA fragment isolated revealed a single, large open-reading frame, designated as SALL4A, that started from a strong consensus initiation sequence and was expected to encode 1053 amino acids. The other splicing variant of SALL4, designated SALL4B, lacked the region corresponding to amino acids 385 to 820 of the full-length SALL4A. The putative protein encoded by SALL4B cDNA was expected to consist of 617 amino acids.

Protocols

Product Specific Protocols
IF protocol for CL Plus 488 SALL4 antibody CL488-24500Download protocol
Standard Protocols
Click here to view our Standard Protocols