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Validation Data Gallery

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  • Western Blot (WB) analysis of mouse liver tissue using SAR1B Polyclonal antibody (22292-1-AP)

    WB analysis of mouse liver using 22292-1-AP (same clone as 22292-1-PBS)

    mouse liver tissue were subjected to SDS PAGE followed by western blot with 22292-1-AP (SAR1B antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 22292-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of mouse small intestine tissue using SAR1B Polyclonal antibody (22292-1-AP)

    WB analysis of mouse small intestine using 22292-1-AP (same clone as 22292-1-PBS)

    mouse small intestine tissue were subjected to SDS PAGE followed by western blot with 22292-1-AP (SAR1B antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 22292-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of mouse brain tissue using SAR1B Polyclonal antibody (22292-1-AP)

    WB analysis of mouse brain using 22292-1-AP (same clone as 22292-1-PBS)

    mouse brain tissue were subjected to SDS PAGE followed by western blot with 22292-1-AP (SAR1B antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 22292-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of mouse liver tissue using SAR1B Polyclonal antibody (22292-1-AP)

    WB analysis of mouse liver using 22292-1-AP (same clone as 22292-1-PBS)

    mouse liver tissue were subjected to SDS PAGE followed by western blot with 22292-1-AP (SAR1B Antibody) at dilution of 1:1000 incubated at 4 degree celsius over night. This data was developed using the same antibody clone with 22292-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of mouse skeletal muscle tissue using SAR1B Polyclonal antibody (22292-1-AP)

    WB analysis of mouse skeletal muscle using 22292-1-AP (same clone as 22292-1-PBS)

    mouse skeletal muscle tissue were subjected to SDS PAGE followed by western blot with 22292-1-AP (SAR1B antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 22292-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of RAW264.7 using SAR1B Polyclonal antibody (22292-1-AP)

    WB analysis of RAW264.7 using 22292-1-AP (same clone as 22292-1-PBS)

    RAW264.7 cells were subjected to SDS PAGE followed by western blot with 22292-1-AP (SAR1B antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 22292-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human liver tissue using SAR1B Polyclonal antibody (22292-1-AP)

    IHC staining of human liver using 22292-1-AP (same clone as 22292-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human liver using 22292-1-AP (SAR1B antibody) at dilution of 1:50 (under 10x lens). This data was developed using the same antibody clone with 22292-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human liver tissue using SAR1B Polyclonal antibody (22292-1-AP)

    IHC staining of human liver using 22292-1-AP (same clone as 22292-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human liver using 22292-1-AP (SAR1B antibody) at dilution of 1:50 (under 40x lens). This data was developed using the same antibody clone with 22292-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human small intestine tissue using SAR1B Polyclonal antibody (22292-1-AP)

    IHC staining of human small intestine using 22292-1-AP (same clone as 22292-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human small intestine using 22292-1-AP (SAR1B antibody) at dilution of 1:50 (under 10x lens). This data was developed using the same antibody clone with 22292-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human small intestine tissue using SAR1B Polyclonal antibody (22292-1-AP)

    IHC staining of human small intestine using 22292-1-AP (same clone as 22292-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human small intestine using 22292-1-AP (SAR1B antibody) at dilution of 1:50 (under 40x lens). This data was developed using the same antibody clone with 22292-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human skeletal muscle tissue using SAR1B Polyclonal antibody (22292-1-AP)

    IHC staining of human skeletal muscle using 22292-1-AP (same clone as 22292-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human skeletal muscle using 22292-1-AP (SAR1B antibody) at dilution of 1:50 (under 10x lens). This data was developed using the same antibody clone with 22292-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human skeletal muscle tissue using SAR1B Polyclonal antibody (22292-1-AP)

    IHC staining of human skeletal muscle using 22292-1-AP (same clone as 22292-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human skeletal muscle using 22292-1-AP (SAR1B antibody) at dilution of 1:50 (under 40x lens). This data was developed using the same antibody clone with 22292-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human skeletal muscle tissue using SAR1B Polyclonal antibody (22292-1-AP)

    IHC staining of human skeletal muscle using 22292-1-AP (same clone as 22292-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human skeletal muscle using 22292-1-AP (SAR1B antibody) at dilution of 1:50 (under 10x lens). This data was developed using the same antibody clone with 22292-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human skeletal muscle tissue using SAR1B Polyclonal antibody (22292-1-AP)

    IHC staining of human skeletal muscle using 22292-1-AP (same clone as 22292-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human skeletal muscle using 22292-1-AP (SAR1B antibody) at dilution of 1:50 (under 40x lens). This data was developed using the same antibody clone with 22292-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human liver tissue using SAR1B Polyclonal antibody (22292-1-AP)

    IHC staining of human liver using 22292-1-AP (same clone as 22292-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human liver using 22292-1-AP (SAR1B antibody) at dilution of 1:50 (under 10x lens). This data was developed using the same antibody clone with 22292-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human liver tissue using SAR1B Polyclonal antibody (22292-1-AP)

    IHC staining of human liver using 22292-1-AP (same clone as 22292-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human liver using 22292-1-AP (SAR1B antibody) at dilution of 1:50 (under 40x lens). This data was developed using the same antibody clone with 22292-1-PBS in a different storage buffer formulation.

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  • Featured Product
  • KD/KO Validated

SAR1B Polyclonal antibody, PBS Only

SAR1B Polyclonal Antibody for WB, IHC, Indirect ELISA
Cat No. 22292-1-PBS

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, Indirect ELISA

Synonyms

Formulation:  PBS Only
PBS and Azide
PBS Only
Conjugate:  Unconjugated
Size/Concentration: 

-/ -

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Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

22292-1-PBS targets SAR1B in WB, IHC, Indirect ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen Peptide 相同性解析による交差性が予測される生物種
Full Name SAR1 homolog B (S. cerevisiae)
Calculated molecular weight 22 kDa
Observed molecular weight 25-27 kDa
GenBank accession numberNM_016103
Gene Symbol SAR1B
Gene ID (NCBI) 51128
RRIDAB_11182713
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDQ9Y6B6
Storage Buffer PBS only , pH 7.3
Storage ConditionsStore at -80°C.

Background Information

SAR1B, also named as SARA2, SARB and GTBPB, belongs to the small GTPase superfamily and SAR1 family. It is involved in transport from the endoplasmic reticulum to the Golgi apparatus. SAR1B is activated by the guanine nucleotide exchange factor PREB. It is involved in the selection of the protein cargo and the assembly of the COPII coat complex. The antibody is specific to SAR1B.