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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

SAT1 Recombinant antibody

SAT1 Recombinant Antibody for WB, IF/ICC, FC (Intra), ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IF/ICC, FC (Intra), ELISA

Conjugate

Unconjugated

CloneNo.

240157G2

Cat no : 83319-1-RR

Synonyms

SSAT, Spermidine/spermine N(1)-acetyltransferase 1, SAT, Polyamine N-acetyltransferase 1, EC:2.3.1.57

Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using SAT1 Recombinant antibody (83319-1-RR)

    WB analysis using 83319-1-RR

    Untreated and DENSPM treated HEK-293 and HeLa cells were subjected to SDS PAGE followed by western blot with 83319-1-RR (SAT1 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of K-562 cells using SAT1 Recombinant antibody (83319-1-RR)

    WB analysis of K-562 using 83319-1-RR

    Various lysates were subjected to SDS PAGE followed by western blot with 83319-1-RR (SAT1 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

  • Immunofluorescence (IF) / fluorescent staining of RAW 264.7 cells using SAT1 Recombinant antibody (83319-1-RR)

    IF Staining of RAW 264.7 using 83319-1-RR

    Immunofluorescent analysis of (4% PFA) fixed RAW 264.7 cells using SAT1 antibody (83319-1-RR, Clone: 240157G2 ) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) (SA00013-2).

  • Flow cytometry (FC) experiment of RAW 264.7 cells using SAT1 Recombinant antibody (83319-1-RR)

    FC experiment of RAW 264.7 using 83319-1-RR

    1x10^6 RAW 264.7 cells were intracellularly stained with 0.25 ug SAT1 Recombinant antibody (83319-1-RR, Clone:240157G2) and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2)(red), or 0.25 ug Rabbit IgG Isotype Control Recombinant Antibody (98136-1-RR, Clone: 240953C9) (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

  • Affinity and Kinetic Characterization of 83319-1-RR

    Affinity and Kinetic Characterization of 83319-1-RR

    Biolayer interferometry (BLl) kinetic assays of 83319-1-RR against Human SAT1 were performed. The affinity constant is 0.484 nM.

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Tested Applications

Positive WB detected inHEK-293 cells, K-562 cells, HeLa cells
Positive IF/ICC detected inRAW 264.7 cells
Positive FC (Intra) detected inRAW 264.7 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:6000
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
Flow Cytometry (FC) (INTRA)FC (INTRA) : 0.25 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

The immunogen of 83319-1-RR is SAT1 Fusion Protein expressed in E. coli.

Tested Reactivity human
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Immunogen SAT1 fusion protein Ag1154 相同性解析による交差性が予測される生物種
Full Name spermidine/spermine N1-acetyltransferase 1
Calculated molecular weight 20 kDa
Observed molecular weight 15-25 kDa
GenBank accession numberBC008424
Gene symbol SAT1
Gene ID (NCBI) 6303
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

Spermidine/spermine N1-acetyltransferase 1 (SAT1 or SSAT1) is the key regulatory enzyme in the catabolism of polyamines, catalyzing acetylation of spermidine or spermine to generate N1-acetyl spermidine or N1-acetyl spermine, and N1, N12-diacetylspermine. The cellular level of SAT1 is normally extremely low, but it is induced rapidly by a variety of stimuli, including polyamines, polyamine analogs, toxic chemicals, certain drugs, and growth factors. Downregulation of SAT1 has been reported in Epstein-Barr virus positive Burkitt's lymphoma cells.

Protocols

Product Specific Protocols
WB protocol for SAT1 antibody 83319-1-RRDownload protocol
IF protocol for SAT1 antibody 83319-1-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols