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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

CoraLite® Plus 488-conjugated SAT1 Recombinant antibody

SAT1 Recombinant Antibody for IF/ICC

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

IF/ICC

Conjugate

CoraLite® Plus 488 Fluorescent Dye

CloneNo.

240157G2

Cat no : CL488-83319

Synonyms

SSAT, Spermidine/spermine N(1)-acetyltransferase 1, SAT, Polyamine N-acetyltransferase 1, EC:2.3.1.57

Validation Data Gallery

Filter:
  • Immunofluorescence (IF) / fluorescent staining of RAW 264.7 cells using CoraLite® Plus 488-conjugated SAT1 Recombinant ant (CL488-83319)

    IF Staining of RAW 264.7 using CL488-83319

    Immunofluorescent analysis of (-20°C Ethanol) fixed RAW 264.7 cells using CoraLite® Plus 488 SAT1 antibody (CL488-83319, Clone: 240157G2 ) at dilution of 1:200.

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Tested Applications

Positive IF/ICC detected inRAW 264.7 cells

Recommended dilution

ApplicationDilution
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

The immunogen of CL488-83319 is SAT1 Fusion Protein expressed in E. coli.

Tested Reactivity human
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Immunogen SAT1 fusion protein Ag1154 相同性解析による交差性が予測される生物種
Full Name spermidine/spermine N1-acetyltransferase 1
Calculated molecular weight 20 kDa
Observed molecular weight 15-25 kDa
GenBank accession numberBC008424
Gene symbol SAT1
Gene ID (NCBI) 6303
Conjugate CoraLite® Plus 488 Fluorescent Dye
Excitation/Emission maxima wavelengths493 nm / 522 nm
Form Liquid
Purification MethodProtein A purification
Storage Buffer PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
Storage ConditionsStore at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

Spermidine/spermine N1-acetyltransferase 1 (SAT1 or SSAT1) is the key regulatory enzyme in the catabolism of polyamines, catalyzing acetylation of spermidine or spermine to generate N1-acetyl spermidine or N1-acetyl spermine, and N1, N12-diacetylspermine. The cellular level of SAT1 is normally extremely low, but it is induced rapidly by a variety of stimuli, including polyamines, polyamine analogs, toxic chemicals, certain drugs, and growth factors. Downregulation of SAT1 has been reported in Epstein-Barr virus positive Burkitt's lymphoma cells.

Protocols

Product Specific Protocols
IF protocol for CL Plus 488 SAT1 antibody CL488-83319Download protocol
Standard Protocols
Click here to view our Standard Protocols