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  • Featured Product
  • KD/KO Validated

CoraLite®594-conjugated SBDS Monoclonal antibody

SBDS Monoclonal Antibody for WB, FC (Intra)

Host / Isotype

Mouse / IgG2a

Reactivity

human, mouse, rat

Applications

WB, FC (Intra)

Conjugate

CoraLite®594 Fluorescent Dye

CloneNo.

1F8A10

Cat no : CL594-67200

Synonyms

SWDS, Shwachman-Bodian-Diamond syndrome protein, SDS, CGI-97, CGI 97

Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using CoraLite®594-conjugated SBDS Monoclonal antibody (CL594-67200)

    WB analysis using CL594-67200

    Various lysates were subjected to SDS PAGE followed by western blot with CL594-67200 (SBDS antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • Flow cytometry (FC) experiment of HeLa cells using CoraLite®594-conjugated SBDS Monoclonal antibody (CL594-67200)

    FC experiment of HeLa using CL594-67200

    1x10^6 HeLa cells were intracellularly stained with 0.4 ug CoraLite®594-conjugated SBDS Monoclonal antibody (CL594-67200, Clone:1F8A10)(red), or 0.4 ug CoraLite®594 Mouse IgG2a isotype control (11A1B2) (CL594-66360-2, Clone: 11A1B2) (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

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Tested Applications

Positive WB detected inHepG2 cells, HeLa cells
Positive FC (Intra) detected inHeLa cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2000
Flow Cytometry (FC) (INTRA)FC (INTRA) : 0.40 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

CL594-67200 targets SBDS in WB, FC (Intra) applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Host / Isotype Mouse / IgG2a
Class Monoclonal
Type Antibody
Immunogen SBDS fusion protein Ag11731 相同性解析による交差性が予測される生物種
Full Name Shwachman-Bodian-Diamond syndrome
Calculated molecular weight 250 aa, 29 kDa
Observed molecular weight 29-31 kDa
GenBank accession numberBC065700
Gene symbol SBDS
Gene ID (NCBI) 51119
Conjugate CoraLite®594 Fluorescent Dye
Excitation/Emission maxima wavelengths588 nm / 604 nm
Form Liquid
Purification MethodProtein A purification
Storage Buffer PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
Storage ConditionsStore at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

Shwachman-Bodian-Diamond syndrome (SBDS) is a member of a highly conserved protein family that exists from archaea to vertebrates and plants. The protein may function in RNA metabolism. Mutations within its gene are associated with Shwachman-Bodian-Diamond syndrome.This gene encodes a member of a highly conserved protein family that exists from archaea to vertebrates and plants. The encoded protein may function in RNA metabolism. Mutations within this gene are associated with Shwachman-Bodian-Diamond syndrome. An alternative transcript has been described, but its biological nature has not been determined. This gene has a closely linked pseudogene that is distally located.

Protocols

Product Specific Protocols
WB protocol for CL594 SBDS antibody CL594-67200Download protocol
FC protocol for CL594 SBDS antibody CL594-67200Download protocol
Standard Protocols
Click here to view our Standard Protocols