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Secretogranin III Polyclonal antibody
Secretogranin III Polyclonal Antibody for IHC, IF-P, ELISA
Host / Isotype
Rabbit / IgG
Reactivity
human, mouse and More (1)
Applications
WB, IHC, IF-P, ELISA
Conjugate
Unconjugated
Cat no : 10954-1-AP
Synonyms
Validation Data Gallery
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Tested Applications
| Positive IHC detected in | mouse pancreas tissue, human pancreas cancer tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Positive IF-P detected in | mouse pancreas tissue |
Recommended dilution
| Application | Dilution |
|---|---|
| Immunohistochemistry (IHC) | IHC : 1:50-1:500 |
| Immunofluorescence (IF)-P | IF-P : 1:50-1:500 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Published Applications
| KD/KO | See 2 publications below |
| WB | See 4 publications below |
| IHC | See 5 publications below |
| IF | See 6 publications below |
Product Information
10954-1-AP targets Secretogranin III in WB, IHC, IF-P, ELISA applications and shows reactivity with human, mouse samples.
| Tested Reactivity | human, mouse |
| Cited Reactivity | human, mouse, rat |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | Secretogranin III fusion protein Ag1397 相同性解析による交差性が予測される生物種 |
| Full Name | secretogranin III |
| Calculated molecular weight | 53 kDa |
| GenBank accession number | BC014539 |
| Gene symbol | Secretogranin 3 |
| Gene ID (NCBI) | 29106 |
| RRID | AB_2190449 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
Background Information
Secretogranin III (SCG3), a member of the granin protein family, is expressed specifically in neuronal and endocrine cells. SCG3 was synthesized as a 61- or 63-kDa protein and processed to a 48-kDa form which, in turn, was partially cleaved to fragments of 28 and 20 kDa.
Protocols
| Product Specific Protocols | |
|---|---|
| IHC protocol for Secretogranin III antibody 10954-1-AP | Download protocol |
| IF protocol for Secretogranin III antibody 10954-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
| Species | Application | Title |
|---|---|---|
J Exp Med Secretogranin III as a disease-associated ligand for antiangiogenic therapy of diabetic retinopathy. | ||
Cancer Lett The oncoprotein HBXIP up-regulates SCG3 through modulating E2F1 and miR-509-3p in hepatoma cells.
| ||
Cereb Cortex Secretogranin III is an astrocyte granin that is overexpressed in reactive glia. | ||
Brain Pathol Secretory sorting receptors carboxypeptidase E and secretogranin III in amyloid β-associated neural degeneration in Alzheimer's disease. | ||
J Endocrinol Secretogranin II binds to secretogranin III and forms secretory granules with orexin, neuropeptide Y, and POMC. | ||
Adv Sci (Weinh) Tuning the Size of Large Dense-Core Vesicles and Quantal Neurotransmitter Release via Secretogranin II Liquid-Liquid Phase Separation.
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