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  • Featured Product
  • KD/KO Validated

CoraLite® Plus 488-conjugated Secretogranin III Polyclonal antibody

Secretogranin III Polyclonal Antibody for IF-P

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

IF-P

Conjugate

CoraLite® Plus 488 Fluorescent Dye

Cat no : CL488-10954

Synonyms

FLJ90833, SCG3, Secretogranin 3, secretogranin III, SGIII

Validation Data Gallery

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  • Immunofluorescence (IF) / fluorescent staining of mouse pancreas tissue using CoraLite® Plus 488-conjugated Secretogranin III Po (CL488-10954)

    IF Staining of mouse pancreas using CL488-10954

    Immunofluorescent analysis of (4% PFA) fixed paraffin-embedded mouse pancreas tissue using CoraLite® Plus 488 Secretogranin III antibody (CL488-10954) at dilution of 1:200. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunofluorescence (IF) / fluorescent staining of mouse pancreas tissue using CoraLite® Plus 488-conjugated Secretogranin III Po (CL488-10954)

    IF Staining of mouse pancreas using CL488-10954

    Immunofluorescent analysis of (4% PFA) fixed paraffin-embedded mouse pancreas tissue using CoraLite® Plus 488 Secretogranin III antibody (CL488-10954) at dilution of 1:200. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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Tested Applications

Positive IF-P detected inmouse pancreas tissue

Recommended dilution

ApplicationDilution
Immunofluorescence (IF)-PIF-P : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

CL488-10954 targets Secretogranin III in IF-P applications and shows reactivity with human, mouse samples.

Tested Reactivity human, mouse
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen Secretogranin III fusion protein Ag1397 相同性解析による交差性が予測される生物種
Full Name secretogranin III
Calculated molecular weight 53 kDa
GenBank accession numberBC014539
Gene symbol Secretogranin 3
Gene ID (NCBI) 29106
Conjugate CoraLite® Plus 488 Fluorescent Dye
Excitation/Emission maxima wavelengths493 nm / 522 nm
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
Storage ConditionsStore at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

Secretogranin III (SCG3), a member of the granin protein family, is expressed specifically in neuronal and endocrine cells. SCG3 was synthesized as a 61- or 63-kDa protein and processed to a 48-kDa form which, in turn, was partially cleaved to fragments of 28 and 20 kDa.

Protocols

Product Specific Protocols
IF protocol for CL Plus 488 Secretogranin III antibody CL488-10954Download protocol
Standard Protocols
Click here to view our Standard Protocols