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Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using CoraLite® Plus 488-conjugated SDC2 Monoclonal anti (CL488-67088)

    WB analysis using CL488-67088

    Various lysates were subjected to SDS PAGE followed by western blot with CL488-67088 (SDC2 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

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CoraLite® Plus 488-conjugated SDC2 Monoclonal antibody

SDC2 Monoclonal Antibody for WB
Cat No. CL488-67088
Clone No.3A7B2

Host / Isotype

Mouse / IgG2a

Reactivity

Human

Applications

WB

Synonyms

Fibroglycan, HSPG, HSPG1, SDC2, SYND2, syndecan 2

Formulation:  PBS and Azide
PBS and Azide
Conjugate:  CoraLite® Plus 488
Size/Concentration: 

-/ -

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テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Positive WB detected inA549 cells, HuH-7 cells, L02 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:4000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

CL488-67088 targets SDC2 in WB applications and shows reactivity with Human samples.

Tested Reactivity Human
Host / Isotype Mouse / IgG2a
Class Monoclonal
Type Antibody
Immunogen SDC2 fusion protein Ag5739 相同性解析による交差性が予測される生物種
Full Name syndecan 2
Calculated molecular weight 22 kDa
Observed molecular weight 22 kDa
GenBank accession numberBC049836
Gene Symbol SDC2
Gene ID (NCBI) 6383
RRIDAB_3084307
Conjugate CoraLite® Plus 488 Fluorescent Dye
Excitation/Emission maxima wavelengths493 nm / 522 nm
Form Liquid
Purification MethodProtein A purification
UNIPROT IDP34741
Storage Buffer PBS with 50% glycerol, 0.05% Proclin300, 0.5% BSA , pH 7.3
Storage ConditionsStore at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

SDC2, containing cell surface heparan sulphate proteoglycan, functions as an integral membrane protein and participates in cell proliferation, cell migration, cell adhesion and signal transduction. It has been reported that the expression level of SDC2 will be up-regulated under hypoxia condition which can induce increased release of cytokines and chemokines. Besides, methylated SDC2 can be used as a potential biomarker for early diagnosis of colon cancer. 67088-1-Ig antibody detects the 19 and 22 kDa SDC2 isoforms as well as the 48 kDa dimeric protein in SDS-PAGE.(PMID: 18803305, 23297089, 30022330, 29945573)

Protocols

Product Specific Protocols
WB protocol for CL Plus 488 SDC2 antibody CL488-67088Download protocol
Standard Protocols
Click here to view our Standard Protocols