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  • Featured Product
  • KD/KO Validated

CoraLite® Plus 488-conjugated SDCCAG3 Polyclonal antibody

SDCCAG3 Polyclonal Antibody for IF/ICC

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

IF/ICC

Conjugate

CoraLite® Plus 488 Fluorescent Dye

Cat no : CL488-15969

Synonyms

Antigen NY CO 3, Endosome-associated-trafficking regulator 1, ENTR1, NY CO 3, SDCCAG3

Validation Data Gallery

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  • Immunofluorescence (IF) / fluorescent staining of HepG2 cells using CoraLite® Plus 488-conjugated SDCCAG3 Polyclonal a (CL488-15969)

    IF Staining of HepG2 using CL488-15969

    Immunofluorescent analysis of (-20°C Ethanol) fixed HepG2 cells using CoraLite® Plus 488 SDCCAG3 antibody (CL488-15969) at dilution of 1:200, CL594-Phalloidin (red).

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Tested Applications

Positive IF/ICC detected inHepG2 cells

Recommended dilution

ApplicationDilution
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

CL488-15969 targets SDCCAG3 in IF/ICC applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen SDCCAG3 fusion protein Ag8745 相同性解析による交差性が予測される生物種
Full Name serologically defined colon cancer antigen 3
Calculated molecular weight 412 aa, 46 kDa
Observed molecular weight 48-60 kDa
GenBank accession numberBC014515
Gene symbol SDCCAG3
Gene ID (NCBI) 10807
Conjugate CoraLite® Plus 488 Fluorescent Dye
Excitation/Emission maxima wavelengths493 nm / 522 nm
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
Storage ConditionsStore at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

SDCCAG3, also named as NY-CO-3, is identified as serologically defined colon cancer antigen-3. It is an endosomal protein which is involved in the regulation of cytokinesis. SDCCAG3 is involved in modulation of TNF response. This gene has four isoforms with MW 40-50 kDa. Observed MW this protein is 48-60 kDa may due to phosphorylation.

Protocols

Product Specific Protocols
IF protocol for CL Plus 488 SDCCAG3 antibody CL488-15969Download protocol
Standard Protocols
Click here to view our Standard Protocols