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  • KD/KO Validated

SDCCAG8 Polyclonal antibody

SDCCAG8 Polyclonal Antibody for IHC, IF/ICC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat, canine, zebrafish

Applications

WB, IHC, IF/ICC, IP, ELISA

Conjugate

Unconjugated

Cat no : 13471-1-AP

Synonyms

NPHP10, HSPC085, hCCCAP, CCCAP, BBS16


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Tested Applications

Positive IHC detected inhuman colon tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inHeLa cells

Recommended dilution

ApplicationDilution
Immunohistochemistry (IHC)IHC : 1:50-1:500
Immunofluorescence (IF)/ICCIF/ICC : 1:400-1:1600
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

13471-1-AP targets SDCCAG8 in WB, IHC, IF/ICC, IP, ELISA applications and shows reactivity with human, mouse, rat, canine, zebrafish samples.

Tested Reactivity human, mouse, rat, canine, zebrafish
Cited Reactivityhuman, mouse, rat, zebrafish
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen SDCCAG8 fusion protein Ag4264 相同性解析による交差性が予測される生物種
Full Name serologically defined colon cancer antigen 8
Calculated molecular weight 713 aa, 83 kDa
Observed molecular weight83 kDa
GenBank accession numberBC032454
Gene symbol SDCCAG8
Gene ID (NCBI) 10806
RRIDAB_2183417
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

SDCCAG8, also named as CCCAP and NY-CO-8, plays a role in the establishment of cell polarity and epithelial lumen formation. It may play a role in ciliogenesis. Loss of SDCCAG8 function as a cause of a retinal-renal ciliopathy and validates exome capture analysis for broadly heterogeneous single-gene disorders. SDCCAG8 is localized at both centrioles and interacts directly with OFD1 (oral-facial-digital syndrome 1), which is associated with NPHP-RC. SDCCAG8 has 4 isoforms with MW 83,78,73 and 41 kDa (refer to UniProt). Catalog#13471-1-AP can recognize all the four isoforms.

Protocols

Product Specific Protocols
IHC protocol for SDCCAG8 antibody 13471-1-APDownload protocol
IF protocol for SDCCAG8 antibody 13471-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanWB

Nature

Global landscape of HIV-human protein complexes.

Authors - Stefanie Jäger
human, zebrafishWB,IF

Nat Genet

Candidate exome capture identifies mutation of SDCCAG8 as the cause of a retinal-renal ciliopathy.

Authors - Otto Edgar A EA
mouseWB, IF, IP

Neuron

SDCCAG8 Regulates Pericentriolar Material Recruitment and Neuronal Migration in the Developing Cortex.

Authors - Ryan Insolera
mouseWB, IHC, IF

J Am Soc Nephrol

Renal-retinal ciliopathy gene sdccag8 regulates DNA damage response signaling.

Authors - Rannar Airik
  • KD Validated
humanIF

J Cell Biol

Centrobin controls primary ciliogenesis in vertebrates.

Authors - Yetunde Adesanya Ogungbenro
mouseWB, IF

Cell Death Dis

Selective loss of RPGRIP1-dependent ciliary targeting of NPHP4, RPGR and SDCCAG8 underlies the degeneration of photoreceptor neurons.

Authors - Patil H H