Validation Data Gallery
Tested Applications
| Positive IF/ICC detected in | MDCK cells |
Recommended dilution
| Application | Dilution |
|---|---|
| Immunofluorescence (IF)/ICC | IF/ICC : 1:50-1:500 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Product Information
CL594-13471 targets SDCCAG8 in IF/ICC applications and shows reactivity with human, mouse, rat, zebrafish, Canine samples.
| Tested Reactivity | human, mouse, rat, zebrafish, Canine |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | SDCCAG8 fusion protein Ag4264 相同性解析による交差性が予測される生物種 |
| Full Name | serologically defined colon cancer antigen 8 |
| Calculated molecular weight | 713 aa, 83 kDa |
| GenBank accession number | BC032454 |
| Gene Symbol | SDCCAG8 |
| Gene ID (NCBI) | 10806 |
| RRID | AB_2919796 |
| Conjugate | CoraLite®594 Fluorescent Dye |
| Excitation/Emission maxima wavelengths | 588 nm / 604 nm |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | Q86SQ7 |
| Storage Buffer | PBS with 50% glycerol, 0.05% Proclin300, 0.5% BSA , pH 7.3 |
| Storage Conditions | Store at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
Background Information
SDCCAG8, also named as CCCAP and NY-CO-8, plays a role in the establishment of cell polarity and epithelial lumen formation. It may play a role in ciliogenesis. Loss of SDCCAG8 function as a cause of a retinal-renal ciliopathy and validates exome capture analysis for broadly heterogeneous single-gene disorders. SDCCAG8 is localized at both centrioles and interacts directly with OFD1 (oral-facial-digital syndrome 1), which is associated with NPHP-RC. SDCCAG8 has 4 isoforms with MW 83,78,73 and 41 kDa (refer to UniProt). Catalog#13471-1-AP can recognize all the four isoforms.
Protocols
| Product Specific Protocols | |
|---|---|
| IF protocol for CL594 SDCCAG8 antibody CL594-13471 | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |