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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

SDCCAG8 Monoclonal antibody

SDCCAG8 Monoclonal Antibody for WB, IHC, ELISA

Host / Isotype

Mouse / IgG2b

Reactivity

human

Applications

WB, IHC, ELISA

Conjugate

Unconjugated

CloneNo.

2H1A4

Cat no : 66284-1-Ig

Synonyms

Antigen NY CO 8, BBS16, CCCAP, hCCCAP, HSPC085, NY CO 8, SDCCAG8

Validation Data Gallery

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  • Western Blot (WB) analysis of HEK-293 cells using SDCCAG8 Monoclonal antibody (66284-1-Ig)

    WB analysis of HEK-293 using 66284-1-Ig

    HEK-293 cells were subjected to SDS PAGE followed by western blot with 66284-1-Ig (SDCCAG8 Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of THP-1 cells using SDCCAG8 Monoclonal antibody (66284-1-Ig)

    WB analysis of THP-1 using 66284-1-Ig

    THP-1 cells were subjected to SDS PAGE followed by western blot with 66284-1-Ig (SDCCAG8 Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • Immunohistochemistry (IHC) staining of human kidney tissue using SDCCAG8 Monoclonal antibody (66284-1-Ig)

    IHC staining of human kidney using 66284-1-Ig

    Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 66284-1-Ig (SDCCAG8 Antibody) at dilution of 1:200 (under 10x lens).

  • Immunohistochemistry (IHC) staining of human kidney tissue using SDCCAG8 Monoclonal antibody (66284-1-Ig)

    IHC staining of human kidney using 66284-1-Ig

    Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 66284-1-Ig (SDCCAG8 Antibody) at dilution of 1:200 (under 40x lens).

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Tested Applications

Positive WB detected inHEK-293 cells, THP-1 cells
Positive IHC detected inhuman kidney tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2000
Immunohistochemistry (IHC)IHC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

66284-1-Ig targets SDCCAG8 in WB, IHC, ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Host / Isotype Mouse / IgG2b
Class Monoclonal
Type Antibody
Immunogen SDCCAG8 fusion protein Ag4264 相同性解析による交差性が予測される生物種
Full Name serologically defined colon cancer antigen 8
Calculated molecular weight 713 aa, 83 kDa
Observed molecular weight 83 kDa
GenBank accession numberBC032454
Gene symbol SDCCAG8
Gene ID (NCBI) 10806
RRIDAB_2881667
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

SDCCAG8, also named as CCCAP and NY-CO-8, plays a role in the establishment of cell polarity and epithelial lumen formation. It may play a role in ciliogenesis. Loss of SDCCAG8 function as a cause of a retinal-renal ciliopathy and validates exome capture analysis for broadly heterogeneous single-gene disorders. SDCCAG8 is localized at both centrioles and interacts directly with OFD1 (oral-facial-digital syndrome 1), which is associated with NPHP-RC.

Protocols

Product Specific Protocols
WB protocol for SDCCAG8 antibody 66284-1-IgDownload protocol
IHC protocol for SDCCAG8 antibody 66284-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols