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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

SDCCAG8 Monoclonal antibody, PBS Only

SDCCAG8 Monoclonal Antibody for WB, IHC, Indirect ELISA

Host / Isotype

Mouse / IgG2b

Reactivity

human

Applications

WB, IHC, Indirect ELISA

Conjugate

Unconjugated

CloneNo.

2H1A4

Cat no : 66284-1-PBS

Synonyms

Antigen NY CO 8, BBS16, CCCAP, hCCCAP, HSPC085, NY CO 8, SDCCAG8

Validation Data Gallery

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  • Western Blot (WB) analysis of HEK-293 cells using SDCCAG8 Monoclonal antibody (66284-1-Ig)

    WB analysis of HEK-293 using 66284-1-Ig (same clone as 66284-1-PBS)

    HEK-293 cells were subjected to SDS PAGE followed by western blot with 66284-1-Ig (SDCCAG8 Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66284-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of THP-1 cells using SDCCAG8 Monoclonal antibody (66284-1-Ig)

    WB analysis of THP-1 using 66284-1-Ig (same clone as 66284-1-PBS)

    THP-1 cells were subjected to SDS PAGE followed by western blot with 66284-1-Ig (SDCCAG8 Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66284-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human kidney tissue using SDCCAG8 Monoclonal antibody (66284-1-Ig)

    IHC staining of human kidney using 66284-1-Ig (same clone as 66284-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 66284-1-Ig (SDCCAG8 Antibody) at dilution of 1:200 (under 10x lens). This data was developed using the same antibody clone with 66284-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human kidney tissue using SDCCAG8 Monoclonal antibody (66284-1-Ig)

    IHC staining of human kidney using 66284-1-Ig (same clone as 66284-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 66284-1-Ig (SDCCAG8 Antibody) at dilution of 1:200 (under 40x lens). This data was developed using the same antibody clone with 66284-1-PBS in a different storage buffer formulation.

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Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

66284-1-PBS targets SDCCAG8 in WB, IHC, Indirect ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Host / Isotype Mouse / IgG2b
Class Monoclonal
Type Antibody
Immunogen SDCCAG8 fusion protein Ag4264 相同性解析による交差性が予測される生物種
Full Name serologically defined colon cancer antigen 8
Calculated molecular weight 713 aa, 83 kDa
Observed molecular weight 83 kDa
GenBank accession numberBC032454
Gene symbol SDCCAG8
Gene ID (NCBI) 10806
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.

Background Information

SDCCAG8, also named as CCCAP and NY-CO-8, plays a role in the establishment of cell polarity and epithelial lumen formation. It may play a role in ciliogenesis. Loss of SDCCAG8 function as a cause of a retinal-renal ciliopathy and validates exome capture analysis for broadly heterogeneous single-gene disorders. SDCCAG8 is localized at both centrioles and interacts directly with OFD1 (oral-facial-digital syndrome 1), which is associated with NPHP-RC.