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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

CoraLite® Plus 488-conjugated SDHA Monoclonal antibody

SDHA Monoclonal Antibody for IF/ICC, FC (Intra)

Host / Isotype

Mouse / IgG1

Reactivity

human, mouse, rat

Applications

IF/ICC, FC (Intra)

Conjugate

CoraLite® Plus 488 Fluorescent Dye

CloneNo.

5E10G12

Cat no : CL488-66588

Synonyms

PPGL5, PGL5, MC2DN1, FP, EC:1.3.5.1

Validation Data Gallery

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  • Immunofluorescence (IF) / fluorescent staining of HepG2 cells using CoraLite® Plus 488-conjugated SDHA Monoclonal anti (CL488-66588)

    IF Staining of HepG2 using CL488-66588

    Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using CoraLite® Plus 488-conjugated SDHA antibody (CL488-66588, Clone: 5E10G12 ) at dilution of 1:100. Red: CL594-Phalloidin stains F-Actin; Blue: DAPI.

  • Immunofluorescence (IF) / fluorescent staining of HepG2 cells using CoraLite® Plus 488-conjugated SDHA Monoclonal anti (CL488-66588)

    IF Staining of HepG2 using CL488-66588

    Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using CL488-66588 (SDHA antibody) at dilution of 1:100.

  • Flow cytometry (FC) experiment of HeLa cells using CoraLite® Plus 488-conjugated SDHA Monoclonal anti (CL488-66588)

    FC experiment of HeLa using CL488-66588

    1X10^6 HeLa cells were intracellularly stained with 0.4 ug CoraLite® Plus 488 Anti-Human SDHA (CL488-66588, Clone:5E10G12) (red), or 0.4 ug Control Antibody. Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

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Tested Applications

Positive IF/ICC detected inHepG2 cells
Positive FC (Intra) detected inHeLa cells

Recommended dilution

ApplicationDilution
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
Flow Cytometry (FC) (INTRA)FC (INTRA) : 0.40 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

CL488-66588 targets SDHA in IF/ICC, FC (Intra) applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Immunogen SDHA fusion protein Ag7023 相同性解析による交差性が予測される生物種
Full Name succinate dehydrogenase complex, subunit A, flavoprotein (Fp)
Calculated molecular weight 73 kDa
Observed molecular weight 62-73 kDa
GenBank accession numberBC001380
Gene symbol SDHA
Gene ID (NCBI) 6389
RRIDAB_2883359
Conjugate CoraLite® Plus 488 Fluorescent Dye
Excitation/Emission maxima wavelengths493 nm / 522 nm
Form Liquid
Purification MethodProtein G purification
Storage Buffer PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
Storage ConditionsStore at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

SDHA, also named as SDH2, SDHF and Fp, belongs to the FAD-dependent oxidoreductase 2 family and FRD/SDH subfamily. SDHA is the flavoprotein subunit of succinate dehydrogenase (SDH) that is involved in complex II of the mitochondrial electron transport chain and is responsible for transferring electrons from succinate to ubiquinone (coenzyme Q). SDHA has 3 isoforms with the molecular mass of 73, 67 and 57 kDa, and the N-terminal (~5 kDa) can be removed in mature form. It can be detected a band of 68 kDa in cutaneous melanoma(PMID:21698147). SDHA may act as a tumor suppressor.

Protocols

Product Specific Protocols
IF protocol for CL Plus 488 SDHA antibody CL488-66588Download protocol
FC protocol for CL Plus 488 SDHA antibody CL488-66588Download protocol
Standard Protocols
Click here to view our Standard Protocols