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Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using SEC13 Recombinant antibody (85110-4-RR)

    WB analysis using 85110-4-RR (same clone as 85110-4-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 85110-4-RR (SEC13 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 85110-4-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of HepG2 cells using SEC13 Recombinant antibody (85110-4-RR)

    IF Staining of HepG2 using 85110-4-RR (same clone as 85110-4-PBS)

    Immunofluorescent analysis of (-20°C Ethanol) fixed HepG2 cells using SEC13 antibody (85110-4-RR, Clone: 242775C3 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2). This data was developed using the same antibody clone with 85110-4-PBS in a different storage buffer formulation.

  • Affinity and Kinetic Characterization of 85110-4-RR

    Affinity and Kinetic Characterization of 85110-4-RR

    Biolayer interferometry (BLl) kinetic assays of 85110-4-RR against Human SEC13 were performed. The affinity constant is 0.114 nM.

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SEC13 Recombinant antibody, PBS Only

SEC13 Uni-rAbTM Recombinant Antibody for WB, IF/ICC, Indirect ELISA
Cat No. 85110-4-PBS
Clone No.242775C3

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IF/ICC, Indirect ELISA

Synonyms

SEC13L1, SEC13A, Protein SEC13 homolog, GATOR2 complex protein SEC13, D3S1231E

Formulation:  PBS Only
PBS and Azide
PBS Only
Conjugate:  Unconjugated
Size/Concentration: 

-/ -

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Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

85110-4-PBS targets SEC13 in WB, IF/ICC, Indirect ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Immunogen SEC13 fusion protein Ag7610 相同性解析による交差性が予測される生物種
Full Name SEC13 homolog (S. cerevisiae)
Calculated molecular weight 36 kDa
Observed molecular weight36 kDa
GenBank accession numberBC002634
Gene Symbol SEC13
Gene ID (NCBI) 6396
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
UNIPROT IDP55735
Storage Buffer PBS only , pH 7.3
Storage ConditionsStore at -80°C.

Background Information

SEC13 is a component of the nuclear pore complex (NPC) and the COPII coat. It is required for esicle biogenesis from endoplasmic reticulum during the transport of proteins. Shuttling of SEC13 between the nucleus and the cytoplasm may couple and regulate functions between these two compartments. (PMID: 14517296; 16407955)