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Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using SEC13 Recombinant antibody (85110-4-RR)

    WB analysis using 85110-4-RR

    Various lysates were subjected to SDS PAGE followed by western blot with 85110-4-RR (SEC13 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

  • Immunofluorescence (IF) / fluorescent staining of HepG2 cells using SEC13 Recombinant antibody (85110-4-RR)

    IF Staining of HepG2 using 85110-4-RR

    Immunofluorescent analysis of (-20°C Ethanol) fixed HepG2 cells using SEC13 antibody (85110-4-RR, Clone: 242775C3 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2).

  • Affinity and Kinetic Characterization of 85110-4-RR

    Affinity and Kinetic Characterization of 85110-4-RR

    Biolayer interferometry (BLl) kinetic assays of 85110-4-RR against Human SEC13 were performed. The affinity constant is 0.114 nM.

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SEC13 Recombinant antibody

SEC13 Uni-rAbTM Recombinant Antibody for WB, IF/ICC, ELISA
Cat No. 85110-4-RR
Clone No.242775C3

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IF/ICC, ELISA

Synonyms

SEC13L1, SEC13A, Protein SEC13 homolog, GATOR2 complex protein SEC13, D3S1231E

Formulation:  PBS and Azide
PBS and Azide
PBS Only
Conjugate:  Unconjugated
Unconjugated
Size/Concentration: 

-/ -

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保証・サポートについて

テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Positive WB detected inLNCaP cells, HEK-293 cells, HepG2 cells, A431 cells, RAW 264.7 cells, mouse pancreas tissue, rat pancreas tissue
Positive IF/ICC detected inHepG2 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:5000-1:50000
Immunofluorescence (IF)/ICCIF/ICC : 1:200-1:800
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

85110-4-RR targets SEC13 in WB, IF/ICC, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Immunogen SEC13 fusion protein Ag7610 相同性解析による交差性が予測される生物種
Full Name SEC13 homolog (S. cerevisiae)
Calculated molecular weight 36 kDa
Observed molecular weight36 kDa
GenBank accession numberBC002634
Gene Symbol SEC13
Gene ID (NCBI) 6396
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
UNIPROT IDP55735
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

SEC13 is a component of the nuclear pore complex (NPC) and the COPII coat. It is required for esicle biogenesis from endoplasmic reticulum during the transport of proteins. Shuttling of SEC13 between the nucleus and the cytoplasm may couple and regulate functions between these two compartments. (PMID: 14517296; 16407955)

Protocols

Product Specific Protocols
WB protocol for SEC13 antibody 85110-4-RRDownload protocol
IF protocol for SEC13 antibody 85110-4-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols