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Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using SEC16B Polyclonal antibody (17245-1-AP)

    WB analysis using 17245-1-AP (same clone as 17245-1-PBS)

    mouse pancreas tissue was subjected to SDS PAGE followed by western blot with 17245-1-AP (SEC16B antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 17245-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human pancreas cancer tissue using SEC16B Polyclonal antibody (17245-1-AP)

    IHC staining of human pancreas cancer using 17245-1-AP (same clone as 17245-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human pancreas cancer tissue slide using 17245-1-AP (SEC16B antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 17245-1-PBS in a different storage buffer formulation.

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SEC16B Polyclonal antibody, PBS Only

SEC16B Polyclonal Antibody for WB, IHC, Indirect ELISA
Cat No. 17245-1-PBS

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IHC, Indirect ELISA

Synonyms

Formulation:  PBS Only
PBS and Azide
PBS Only
Conjugate:  Unconjugated
Size/Concentration: 

-/ -

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Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

17245-1-PBS targets SEC16B in WB, IHC, Indirect ELISA applications and shows reactivity with human, mouse samples.

Tested Reactivity human, mouse
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen SEC16B fusion protein Ag11173 相同性解析による交差性が予測される生物種
Full Name SEC16 homolog B (S. cerevisiae)
Calculated molecular weight 71 kDa, 117 kDa
Observed molecular weight 60-70 kDa
GenBank accession numberBC009106
Gene Symbol SEC16B
Gene ID (NCBI) 89866
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDQ96JE7
Storage Buffer PBS only , pH 7.3
Storage ConditionsStore at -80°C.

Background Information

SEC16B also known as LZTR2, RGPR-p117, is a mammalian homolog of S. cerevisiae Sec16 that is required for organization of transitional endoplasmic reticulum (ER) sites and protein export (PMID: 17192411). SEC16B regulates the transport of peroxisomal biogenesis factors PEX3 and PEX16 from the ER to peroxisomes (PMID:21768384). SEC16B may be involved in human obesity to possess an association between single nucleotide polymorphisms and different measures of obesity (PMID: 28413634).