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Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using SEC24A Recombinant antibody (82908-1-RR)

    WB analysis using 82908-1-RR

    Various lysates were subjected to SDS PAGE followed by western blot with 82908-1-RR (SEC24A antibody) at dilution of 1:2500 incubated at room temperature for 1.5 hours.

  • Immunofluorescence (IF) / fluorescent staining of HeLa cells using SEC24A Recombinant antibody (82908-1-RR)

    IF Staining of HeLa using 82908-1-RR

    Immunofluorescent analysis of (-20°C Ethanol) fixed HeLa cells using SEC24A antibody (82908-1-RR, Clone: 230105B3 ) at dilution of 1:250 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2).

  • Flow cytometry (FC) experiment of HepG2 cells using SEC24A Recombinant antibody (82908-1-RR)

    FC experiment of HepG2 using 82908-1-RR

    1x10^6 HepG2 cells were intracellularly stained with 0.25 ug Anti-Human SEC24A (82908-1-RR, Clone:230105B3) and APC-Conjugated Goat Anti-Rabbit IgG(H+L) at dilution 1:166 (red), or 0.25 ug Rabbit IgG control Rabbit PolyAb (30000-0-AP, Clone: ) (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

  • Affinity and Kinetic Characterization of 82908-1-RR

    Affinity and Kinetic Characterization of 82908-1-RR

    Biolayer interferometry (BLl) kinetic assays of 82908-1-RR against Human SEC24A were performed. The affinity constant is below 1 pM.

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SEC24A Recombinant antibody

SEC24A Uni-rAbTM Recombinant Antibody for WB, IF/ICC, FC (Intra), ELISA
Cat No. 82908-1-RR
Clone No.230105B3

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IF/ICC, FC (Intra), ELISA

Synonyms

SEC24-related protein A, Protein transport protein Sec24A, 230105B3

Formulation:  PBS and Azide
PBS and Azide
PBS Only
Conjugate:  Unconjugated
Unconjugated
CoraLite® Plus 488
Size/Concentration: 

-/ -

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保証・サポートについて

テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Positive WB detected inU-87 MG cells, HEK-293 cells, HeLa cells, HepG2 cells
Positive IF/ICC detected inHeLa cells
Positive FC (Intra) detected inHepG2 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:5000
Immunofluorescence (IF)/ICCIF/ICC : 1:125-1:500
Flow Cytometry (FC) (INTRA)FC (INTRA) : 0.25 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

82908-1-RR targets SEC24A in WB, IF/ICC, FC (Intra), ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Immunogen SEC24A fusion protein Ag8723 相同性解析による交差性が予測される生物種
Full Name SEC24 family, member A (S. cerevisiae)
Calculated molecular weight 613 aa, 66 kDa
Observed molecular weight120 kDa
GenBank accession numberBC019341
Gene Symbol SEC24A
Gene ID (NCBI) 10802
RRIDAB_3670637
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
UNIPROT IDO95486
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

COPII-coated vesicles mediate anterograde transport from the ER to the Golgi apparatus. COPII is composed of three parts: two coat protein complexes (Sec23/24 complex and Sec13/31 complex) and one small GTP-binding protein, Sar1 (PMID: 9568718). Sec24 subunit of the coat complex has been implicated in cargo recognition (PMID: 17316621). In mammal cells, there are four Sec24 isoforms, termed Sec24A, Sec24B, Sec24C, and Sec24D (PMID: 10329445). This antibody is specific to SEC24A.

Protocols

Product Specific Protocols
WB protocol for SEC24A antibody 82908-1-RRDownload protocol
IF protocol for SEC24A antibody 82908-1-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols