Validation Data Gallery
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Tested Applications
| Positive IF/ICC detected in | HeLa cells |
| Positive FC (Intra) detected in | Hela cells |
Recommended dilution
| Application | Dilution |
|---|---|
| Immunofluorescence (IF)/ICC | IF/ICC : 1:50-1:500 |
| Flow Cytometry (FC) (INTRA) | FC (INTRA) : 0.80 ug per 10^6 cells in a 100 µl suspension |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Product Information
CL488-82908 targets SEC24A in IF/ICC, FC (Intra) applications and shows reactivity with human samples.
| Tested Reactivity | human |
| Host / Isotype | Rabbit / IgG |
| Class | Recombinant |
| Type | Antibody |
| Immunogen | SEC24A fusion protein Ag8723 相同性解析による交差性が予測される生物種 |
| Full Name | SEC24 family, member A (S. cerevisiae) |
| Calculated molecular weight | 613 aa, 66 kDa |
| Observed molecular weight | 120 kDa |
| GenBank accession number | BC019341 |
| Gene Symbol | SEC24A |
| Gene ID (NCBI) | 10802 |
| RRID | AB_3673109 |
| Conjugate | CoraLite® Plus 488 Fluorescent Dye |
| Excitation/Emission maxima wavelengths | 493 nm / 522 nm |
| Form | Liquid |
| Purification Method | Protein A purification |
| UNIPROT ID | O95486 |
| Storage Buffer | PBS with 50% glycerol, 0.05% Proclin300, 0.5% BSA , pH 7.3 |
| Storage Conditions | Store at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
Background Information
COPII-coated vesicles mediate anterograde transport from the ER to the Golgi apparatus. COPII is composed of three parts: two coat protein complexes (Sec23/24 complex and Sec13/31 complex) and one small GTP-binding protein, Sar1 (PMID: 9568718). Sec24 subunit of the coat complex has been implicated in cargo recognition (PMID: 17316621). In mammal cells, there are four Sec24 isoforms, termed Sec24A, Sec24B, Sec24C, and Sec24D (PMID: 10329445). This antibody is specific to SEC24A.
Protocols
| Product Specific Protocols | |
|---|---|
| IF protocol for CL Plus 488 SEC24A antibody CL488-82908 | Download protocol |
| FC protocol for CL Plus 488 SEC24A antibody CL488-82908 | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |