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Species Reactivity
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Species Reactivity
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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

CoraLite® Plus 488-conjugated SEC24A Recombinant antibody

SEC24A Recombinant Antibody for IF/ICC, FC (Intra)

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

IF/ICC, FC (Intra)

Conjugate

CoraLite® Plus 488 Fluorescent Dye

CloneNo.

230105B3

Cat no : CL488-82908

Synonyms

SEC24-related protein A, Protein transport protein Sec24A, 230105B3

Validation Data Gallery

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  • Immunofluorescence (IF) / fluorescent staining of HeLa cells using CoraLite® Plus 488-conjugated SEC24A Recombinant a (CL488-82908)

    IF Staining of HeLa using CL488-82908

    Immunofluorescent analysis of (4% PFA) fixed HeLa cells using CoraLite® Plus 488 SEC24A antibody (CL488-82908, Clone: 230105B3 ) at dilution of 1:200.

  • Flow cytometry (FC) experiment of Hela cells using CoraLite® Plus 488-conjugated SEC24A Recombinant a (CL488-82908)

    FC experiment of Hela using CL488-82908

    1x10^6 Hela cells were intracellularly stained with 0.8 ug Coralite® Plus 488 Sec24a Recombinant Antibody (CL488-82908, Clone:230105B3)(red), or 0.8 ug CoraLite® Plus 488-conjugated Rabbit IgG control Rabbit PolyAb (CL488-30000) (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

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Tested Applications

Positive IF/ICC detected inHeLa cells
Positive FC (Intra) detected inHela cells

Recommended dilution

ApplicationDilution
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
Flow Cytometry (FC) (INTRA)FC (INTRA) : 0.80 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

CL488-82908 targets SEC24A in IF/ICC, FC (Intra) applications and shows reactivity with human samples.

Tested Reactivity human
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Immunogen SEC24A fusion protein Ag8723 相同性解析による交差性が予測される生物種
Full Name SEC24 family, member A (S. cerevisiae)
Calculated molecular weight 613 aa, 66 kDa
Observed molecular weight120 kDa
GenBank accession numberBC019341
Gene symbol SEC24A
Gene ID (NCBI) 10802
RRIDAB_3673109
Conjugate CoraLite® Plus 488 Fluorescent Dye
Excitation/Emission maxima wavelengths493 nm / 522 nm
Form Liquid
Purification MethodProtein A purification
Storage Buffer PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
Storage ConditionsStore at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

COPII-coated vesicles mediate anterograde transport from the ER to the Golgi apparatus. COPII is composed of three parts: two coat protein complexes (Sec23/24 complex and Sec13/31 complex) and one small GTP-binding protein, Sar1 (PMID: 9568718). Sec24 subunit of the coat complex has been implicated in cargo recognition (PMID: 17316621). In mammal cells, there are four Sec24 isoforms, termed Sec24A, Sec24B, Sec24C, and Sec24D (PMID: 10329445). This antibody is specific to SEC24A.

Protocols

Product Specific Protocols
IF protocol for CL Plus 488 SEC24A antibody CL488-82908Download protocol
FC protocol for CL Plus 488 SEC24A antibody CL488-82908Download protocol
Standard Protocols
Click here to view our Standard Protocols