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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

SEC24D Monoclonal antibody

SEC24D Monoclonal Antibody for WB, IF/ICC, ELISA

Host / Isotype

Mouse / IgG2a

Reactivity

human

Applications

WB, IF/ICC, ELISA

Conjugate

Unconjugated

CloneNo.

3G5G9

Cat no : 67409-1-Ig

Synonyms

SEC24-related protein D, SEC24 related protein D, KIAA0755, 3G5G9

Validation Data Gallery

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  • Western Blot (WB) analysis of Jurkat cells using SEC24D Monoclonal antibody (67409-1-Ig)

    WB analysis of Jurkat using 67409-1-Ig

    Jurkat cells were subjected to SDS PAGE followed by western blot with 67409-1-Ig (SEC24D antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of HepG2 cells using SEC24D Monoclonal antibody (67409-1-Ig)

    WB analysis of HepG2 using 67409-1-Ig

    HepG2 cells were subjected to SDS PAGE followed by western blot with 67409-1-Ig (SEC24D antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of HeLa cells using SEC24D Monoclonal antibody (67409-1-Ig)

    WB analysis of HeLa using 67409-1-Ig

    HeLa cells were subjected to SDS PAGE followed by western blot with 67409-1-Ig (SEC24D antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

  • Immunofluorescence (IF) / fluorescent staining of HepG2 cells using SEC24D Monoclonal antibody (67409-1-Ig)

    IF Staining of HepG2 using 67409-1-Ig

    Immunofluorescent analysis of (-20°C Ethanol) fixed HepG2 cells using SEC24D antibody (67409-1-Ig, Clone: 3G5G9 ) at dilution of 1:400 and Multi-rAb CoraLite ® Plus 488-Goat Anti-Mouse Recombinant Secondary Antibody (H+L) (RGAM002), CL594-Phalloidin (red).

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Tested Applications

Positive WB detected inJurkat cells, HepG2 cells, HeLa cells
Positive IF/ICC detected inHepG2 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:2000-1:8000
Immunofluorescence (IF)/ICCIF/ICC : 1:200-1:800
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

67409-1-Ig targets SEC24D in WB, IF/ICC, ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Host / Isotype Mouse / IgG2a
Class Monoclonal
Type Antibody
Immunogen SEC24D fusion protein Ag4657 相同性解析による交差性が予測される生物種
Full Name SEC24 family, member D (S. cerevisiae)
Calculated molecular weight 1033 aa, 113 kDa
Observed molecular weight 113 kDa
GenBank accession numberBC035761
Gene symbol SEC24D
Gene ID (NCBI) 9871
RRIDAB_2882650
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

COPII-coated vesicles mediate anterograde transport from the ER to the Golgi apparatus. COPII is composed of three parts: two coat protein complexes (Sec23/24 complex and Sec13/31 complex) and one small GTP-binding protein, Sar1 (PMID: 9568718). Sec24 subunit of the coat complex has been implicated in cargo recognition (PMID: 17316621). In mammal cells there are four Sec24 isoforms, termed Sec24A, Sec24B, Sec24C, and Sec24D (PMID: 10329445).

Protocols

Product Specific Protocols
WB protocol for SEC24D antibody 67409-1-IgDownload protocol
IF protocol for SEC24D antibody 67409-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols