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Validation Data Gallery

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  • Immunofluorescence (IF) / fluorescent staining of HepG2 cells using CoraLite®594-conjugated SEC24D Monoclonal antibody (CL594-67409)

    IF Staining of HepG2 using CL594-67409

    Immunofluorescent analysis of (-20°C Methanol) fixed HepG2 cells using CoraLite®594 SEC24D antibody (CL594-67409, Clone: 3G5G9 ) at dilution of 1:100. DAPI (blue).

  • Immunofluorescence (IF) / fluorescent staining of HepG2 cells using CoraLite®594-conjugated SEC24D Monoclonal antibody (CL594-67409)

    IF Staining of HepG2 using CL594-67409

    Immunofluorescent analysis of (-20°C Ethanol) fixed HepG2 cells using CoraLite®594 SEC24D antibody (CL594-67409, Clone: 3G5G9 ) at dilution of 1:200.

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CoraLite®594-conjugated SEC24D Monoclonal antibody

SEC24D Monoclonal Antibody for IF/ICC
Cat No. CL594-67409
Clone No.3G5G9

Host / Isotype

Mouse / IgG2a

Reactivity

human

Applications

IF/ICC

Synonyms

SEC24-related protein D, SEC24 related protein D, KIAA0755, 3G5G9

Formulation:  PBS and Azide
PBS and Azide
Conjugate:  CoraLite®594
Size/Concentration: 

-/ -

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国内在庫・納期について

約2万点のプロテインテック製品をコスモバイオ社物流センター（国内）に在庫しています。国内在庫の有無はコスモバイオ社ホームページの「品番検索」でカタログ番号を検索して確認できます。

保証・サポートについて

テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Positive IF/ICC detected inHepG2 cells

Recommended dilution

ApplicationDilution
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

CL594-67409 targets SEC24D in IF/ICC applications and shows reactivity with human samples.

Tested Reactivity human
Host / Isotype Mouse / IgG2a
Class Monoclonal
Type Antibody
Immunogen SEC24D fusion protein Ag4657 相同性解析による交差性が予測される生物種
Full Name SEC24 family, member D (S. cerevisiae)
Calculated molecular weight 1033 aa, 113 kDa
Observed molecular weight 113 kDa
GenBank accession numberBC035761
Gene Symbol SEC24D
Gene ID (NCBI) 9871
RRIDAB_2920127
Conjugate CoraLite®594 Fluorescent Dye
Excitation/Emission maxima wavelengths588 nm / 604 nm
Form Liquid
Purification MethodProtein A purification
UNIPROT IDO94855
Storage Buffer PBS with 50% glycerol, 0.05% Proclin300, 0.5% BSA , pH 7.3
Storage ConditionsStore at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

COPII-coated vesicles mediate anterograde transport from the ER to the Golgi apparatus. COPII is composed of three parts: two coat protein complexes (Sec23/24 complex and Sec13/31 complex) and one small GTP-binding protein, Sar1 (PMID: 9568718). Sec24 subunit of the coat complex has been implicated in cargo recognition (PMID: 17316621). In mammal cells there are four Sec24 isoforms, termed Sec24A, Sec24B, Sec24C, and Sec24D (PMID: 10329445).

Protocols

Product Specific Protocols
IF protocol for CL594 SEC24D antibody CL594-67409Download protocol
Standard Protocols
Click here to view our Standard Protocols