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  • Featured Product
  • KD/KO Validated

CoraLite®594-conjugated SEC5/EXOC2 Monoclonal antibody

SEC5/EXOC2 Monoclonal Antibody for IF/ICC

Host / Isotype

Mouse / IgG2b

Reactivity

human, mouse, pig, rat

Applications

IF/ICC

Conjugate

CoraLite®594 Fluorescent Dye

CloneNo.

1C11G2

Cat no : CL594-66011

Synonyms

EXOC2, exocyst complex component 2, Exocyst complex component Sec5, FLJ11026, SEC5, SEC5L1, Sec5p

Validation Data Gallery

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  • Immunofluorescence (IF) / fluorescent staining of HeLa cells using CoraLite®594-conjugated SEC5/EXOC2 Monoclonal anti (CL594-66011)

    IF Staining of HeLa using CL594-66011

    Immunofluorescent analysis of (-20°C Ethanol) fixed HeLa cells using CL594-66011 (SEC5/EXOC2 antibody) at dilution of 1:100.

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Tested Applications

Positive IF/ICC detected inHeLa cells

Recommended dilution

ApplicationDilution
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

The immunogen of CL594-66011 is SEC5/EXOC2 Fusion Protein expressed in E. coli.

Tested Reactivity human, mouse, pig, rat
Host / Isotype Mouse / IgG2b
Class Monoclonal
Type Antibody
Immunogen SEC5/EXOC2 fusion protein Ag17866 相同性解析による交差性が予測される生物種
Full Name exocyst complex component 2
Calculated molecular weight 924 aa, 104 kDa
Observed molecular weight 100 kDa
GenBank accession numberBC016918
Gene symbol SEC5
Gene ID (NCBI) 55770
RRIDAB_2883476
Conjugate CoraLite®594 Fluorescent Dye
Excitation/Emission maxima wavelengths588 nm / 604 nm
Form Liquid
Purification MethodProtein A purification
Storage Buffer PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
Storage ConditionsStore at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

EXOC2 (exocyst complex component 2), also known as SEC5 and SEC5L1, is a component of the exocyst complex, and is required to mediate RalB-dependent survival signals in transformed cells. The exocyst complex, composed of eight evolutionarily conserved subunits (SEC3, SEC5, SEC6, SEC8, SEC10, SEC15, EXO70, and EXO84), is involved in tethering post-Golgi secretory vesicles to specific plasma membrane domains. The gene of EXOC2 maps to chromosome 6p25.3, and encodes a 924-amino acid protein with an experimentally determined molecular mass of 95-100 kDa. EXOC2 mRNA is widely expressed with highest levels in brain and placenta.

Protocols

Product Specific Protocols
IF protocol for CL594 SEC5/EXOC2 antibody CL594-66011Download protocol
Standard Protocols
Click here to view our Standard Protocols