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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

CoraLite® Plus 488-conjugated SEC61B Polyclonal antibody

SEC61B Polyclonal Antibody for IF/ICC

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

IF/ICC

Conjugate

CoraLite® Plus 488 Fluorescent Dye

Cat no : CL488-15087

Synonyms

Sec61 beta subunit, SEC61B

Validation Data Gallery

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  • Immunofluorescence (IF) / fluorescent staining of HepG2 cells using CoraLite® Plus 488-conjugated SEC61B Polyclonal an (CL488-15087)

    IF Staining of HepG2 using CL488-15087

    Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using CoraLite® Plus 488 SEC61B antibody (CL488-15087) at dilution of 1:200.

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Tested Applications

Positive IF/ICC detected inHepG2 cells

Recommended dilution

ApplicationDilution
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

CL488-15087 targets SEC61B in IF/ICC applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen SEC61B fusion protein Ag7169 相同性解析による交差性が予測される生物種
Full Name Sec61 beta subunit
Calculated molecular weight 10 kDa
Observed molecular weight 10-15 kDa
GenBank accession numberBC001734
Gene symbol SEC61B
Gene ID (NCBI) 10952
Conjugate CoraLite® Plus 488 Fluorescent Dye
Excitation/Emission maxima wavelengths493 nm / 522 nm
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
Storage ConditionsStore at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

The heteromeric SEC61 complex is composed of alpha, beta and gamma subunits. Oligomers of the SEC61 complex form a transmembrane channel where proteins are translocated across and integrated into the ER membrane (PMID: 10212142). SEC61B is the beta subunit of the SEC61 complex. It has been reported that SEC61B facilitates cotranslational protein transport and interacts with the signal peptidase during translocation (PMID: 9585408).

Protocols

Product Specific Protocols
IF protocol for CL Plus 488 SEC61B antibody CL488-15087Download protocol
Standard Protocols
Click here to view our Standard Protocols