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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

SECTM1 Monoclonal antibody

SECTM1 Monoclonal Antibody for WB, IHC, ELISA

Host / Isotype

Mouse / IgM

Reactivity

human

Applications

WB, IHC, ELISA

Conjugate

Unconjugated

CloneNo.

1C11B4

Cat no : 60281-1-Ig

Synonyms

K12, Protein K 12, secreted and transmembrane 1, SECTM1

Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using SECTM1 Monoclonal antibody (60281-1-Ig)

    WB analysis using 60281-1-Ig

    Various lysates were subjected to SDS PAGE followed by western blot with 60281-1-Ig (SECTM1 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours. The membrane was stripped and reblotted with HRP-conjugated GAPDH Monoclonal antibody (HRP-60004) as loading control.

  • Immunohistochemistry (IHC) staining of human breast cancer tissue using SECTM1 Monoclonal antibody (60281-1-Ig)

    IHC staining of human breast cancer using 60281-1-Ig

    Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 60281-1-Ig (SECTM1 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human breast cancer tissue using SECTM1 Monoclonal antibody (60281-1-Ig)

    IHC staining of human breast cancer using 60281-1-Ig

    Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 60281-1-Ig (SECTM1 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human colon cancer tissue using SECTM1 Monoclonal antibody (60281-1-Ig)

    IHC staining of human colon cancer using 60281-1-Ig

    Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 60281-1-Ig (SECTM1 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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Tested Applications

Positive WB detected inHaCaT cells, JAR cells, NCCIT cells, PC-3 cells, LNCaP cells, HeLa cells, K-562 cells
Positive IHC detected inhuman breast cancer tissue, human colon cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:4000
Immunohistochemistry (IHC)IHC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

60281-1-Ig targets SECTM1 in WB, IHC, ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Cited Reactivityhuman
Host / Isotype Mouse / IgM
Class Monoclonal
Type Antibody
Immunogen SECTM1 fusion protein Ag14645 相同性解析による交差性が予測される生物種
Full Name secreted and transmembrane 1
Calculated molecular weight 248 aa, 27 kDa
Observed molecular weight 27 kDa
GenBank accession numberBC017716
Gene symbol SECTM1
Gene ID (NCBI) 6398
RRIDAB_2881399
Conjugate Unconjugated
Form Liquid
Purification MethodThiophilic affinity chromatograph
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

SECTM1, also named as protein K12, is originally identified by its location just upstream of the CD7 locus. It has been proposed as a ligand for CD7. SECTM1 is involved in thymocyte signaling. This antibody is specific to SECTM1.

Protocols

Product Specific Protocols
WB protocol for SECTM1 antibody 60281-1-IgDownload protocol
IHC protocol for SECTM1 antibody 60281-1-IgDownload protocol
FC protocol for SECTM1 antibody 60281-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanWB,IHC

iScience

SECTM1 is upregulated in immuno-hot tumors and predicts immunotherapeutic efficacy in multiple cancers

Authors - Jie Mei
View Article