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Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using SECTM1 Monoclonal antibody (60281-1-Ig)

    WB analysis using 60281-1-Ig (same clone as 60281-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 60281-1-Ig (SECTM1 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours. The membrane was stripped and reblotted with HRP-conjugated GAPDH Monoclonal antibody (HRP-60004) as loading control. This data was developed using the same antibody clone with 60281-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human breast cancer tissue using SECTM1 Monoclonal antibody (60281-1-Ig)

    IHC staining of human breast cancer using 60281-1-Ig (same clone as 60281-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 60281-1-Ig (SECTM1 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 60281-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human breast cancer tissue using SECTM1 Monoclonal antibody (60281-1-Ig)

    IHC staining of human breast cancer using 60281-1-Ig (same clone as 60281-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 60281-1-Ig (SECTM1 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 60281-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human colon cancer tissue using SECTM1 Monoclonal antibody (60281-1-Ig)

    IHC staining of human colon cancer using 60281-1-Ig (same clone as 60281-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 60281-1-Ig (SECTM1 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 60281-1-PBS in a different storage buffer formulation.

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SECTM1 Monoclonal antibody, PBS Only

SECTM1 Monoclonal Antibody for WB, IHC, Indirect ELISA
Cat No. 60281-1-PBS
Clone No.1C11B4

Host / Isotype

Mouse / IgM

Reactivity

human

Applications

WB, IHC, Indirect ELISA

Synonyms

K12, Protein K 12, secreted and transmembrane 1, SECTM1

Formulation:  PBS Only
PBS and Azide
PBS Only
Conjugate:  Unconjugated
Size/Concentration: 

-/ -

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テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Mouse monoclonal antibodies of IgM isotype can be detected with "anti-mouse IgG (H+L)" secondary antibodies.

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

60281-1-PBS targets SECTM1 in WB, IHC, Indirect ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Host / Isotype Mouse / IgM
Class Monoclonal
Type Antibody
Immunogen SECTM1 fusion protein Ag14645 相同性解析による交差性が予測される生物種
Full Name secreted and transmembrane 1
Calculated molecular weight 248 aa, 27 kDa
Observed molecular weight 27 kDa
GenBank accession numberBC017716
Gene Symbol SECTM1
Gene ID (NCBI) 6398
RRIDAB_2881399
Conjugate Unconjugated
Form Liquid
Purification MethodThiophilic affinity chromatograph
UNIPROT IDQ8WVN6
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.

Background Information

SECTM1, also named as protein K12, is originally identified by its location just upstream of the CD7 locus. It has been proposed as a ligand for CD7. SECTM1 is involved in thymocyte signaling. This antibody is specific to SECTM1.