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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

FcZero-rAb™ PE Anti-Human SELPLG/CD162 Rabbit Recombinant Antibody

SELPLG/CD162 Recombinant Antibody for FC

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

FC

Conjugate

PE Fluorescent Dye

CloneNo.

241297F10

Cat no : PE-FcA98084

Synonyms

SELPLG, PSGL1, PSGL 1, P-selectin glycoprotein ligand 1, CLA

Validation Data Gallery

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  • Flow cytometry (FC) experiment of human peripheral blood leukocyte using FcZero-rAb™ PE Anti-Human SELPLG/CD162 Rabbit Reco (PE-FcA98084)

    FC experiment of human peripheral blood leukocyte using PE-FcA98084

    1x10^6 human peripheral blood leukocytes were surface stained with 5 ul PE Anti-Human SELPLG/CD162 Rabbit Recombinant Antibody (PE-FcA98084, Clone: 241297F10) or 5 ul PE Rabbit IgG Isotype Control Recombinant Antibody (PE-FcA98136, Clone: 240953C9). Cells were not fixed.

  • Flow cytometry (FC) experiment of human peripheral blood leukocyte using FcZero-rAb™ PE Anti-Human SELPLG/CD162 Rabbit Reco (PE-FcA98084)

    FC experiment of human peripheral blood leukocyte using PE-FcA98084

    1x10^6 human peripheral blood leukocytes were surface stained with 5 ul PE Anti-Human SELPLG/CD162 Rabbit Recombinant Antibody (PE-FcA98084, Clone: 241297F10) (red) or 5 ul PE Rabbit IgG Isotype Control Recombinant Antibody (PE-FcA98136, Clone: 240953C9) (blue). Cells were not fixed.

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Tested Applications

Positive FC detected inhuman peripheral blood leukocytes

Recommended dilution

ApplicationDilution
This reagent has been pre-titrated and tested for flow cytometric analysis. The suggested use of this reagent is 5 ul per 10^6 cells in a 100 µl suspension or 5 ul per 100 µl of whole blood.
Sample-dependent, Check data in validation data gallery.

Product Information

PE-FcA98084 targets SELPLG/CD162 in FC applications and shows reactivity with human samples.

Tested Reactivity human
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Immunogen Recombinant Protein 相同性解析による交差性が予測される生物種
Full Name selectin P ligand
Calculated molecular weight 412 aa, 43 kDa
GenBank accession numberBC029782
Gene symbol SELPLG
Gene ID (NCBI) 6404
Conjugate PE Fluorescent Dye
Excitation/Emission maxima wavelengths496 nm, 565 nm / 578 nm
Form Liquid
Purification MethodProtein A purification
Storage Buffer PBS with 0.09% sodium azide and 0.5% BSA.
Storage ConditionsStore at 2-8°C. Avoid exposure to light. Stable for one year after shipment.

Background Information

SELPLG (selectin P ligand), also known as CD162 or PSGL-1, is a 120 kDa type I transmembrane mucin-like glycoprotein. It is an adhesion molecule expressed on the surface of neutrophils, monocytes, and most lymphocytes as a homodimer of approximately 230-250 kDa (PMID: 7545173; 1378449; 9353350). SELPLG plays a critical role in the early stages of inflammation due to its ability to bind E-selectin (CD62E), L-selectin (CD62L), and P-selectin (CD62P) (PMID: 35597982).

Protocols

Product Specific Protocols
FC protocol for PE SELPLG/CD162 antibody PE-FcA98084Download protocol
Standard Protocols
Click here to view our Standard Protocols