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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

SEMA7A Monoclonal antibody

SEMA7A Monoclonal Antibody for WB, ELISA

Host / Isotype

Mouse / IgG2b

Reactivity

Human and More (1)

Applications

WB, ELISA

Conjugate

Unconjugated

CloneNo.

3A5G1

Cat no : 67397-1-Ig

Synonyms

CD108, CDw108, H SEMA K1, H Sema L, JMH, JMH blood group antigen, Sema K1, Sema L, SEMA7A, SEMAK1, SEMAL, Semaphorin 7A, Semaphorin K1, Semaphorin L

Validation Data Gallery

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  • Western Blot (WB) analysis of human red blood cells using SEMA7A Monoclonal antibody (67397-1-Ig)

    WB analysis of human red blood cells using 67397-1-Ig

    human red blood cells were subjected to SDS PAGE followed by western blot with 67397-1-Ig (SEMA7A antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of human placenta tissue using SEMA7A Monoclonal antibody (67397-1-Ig)

    WB analysis of human placenta using 67397-1-Ig

    human placenta tissue were subjected to SDS PAGE followed by western blot with 67397-1-Ig (SEMA7A antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

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Tested Applications

Positive WB detected inhuman red blood cells, human placenta tissue

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:2000-1:6000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Published Applications

WBSee 1 publications below

Product Information

67397-1-Ig targets SEMA7A in WB, ELISA applications and shows reactivity with Human samples.

Tested Reactivity Human
Cited Reactivity mouse
Host / Isotype Mouse / IgG2b
Class Monoclonal
Type Antibody
Immunogen SEMA7A fusion protein Ag28804 相同性解析による交差性が予測される生物種
Full Name semaphorin 7A, GPI membrane anchor (John Milton Hagen blood group)
Calculated molecular weight 666 aa, 75 kDa
Observed molecular weight 75-80 kDa
GenBank accession numberBC101643
Gene symbol SEMA7A
Gene ID (NCBI) 8482
RRIDAB_2882640
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

SEMA7A, also named CD108 and SEMAL, belongs to the semaphorin family. SEMA7A is the only membrane‐associated glycosylphosphatidylinositol (GPI)‐linked semaphorin and plays a role in the regulation of neuronal axon outgrowth. In addition to its role in guiding axon pathfinding during neuronal development, Sema7A has diverse functions in morphogenesis and immune cell control and regulates T cell responses via the α1β1 integrin receptor (PMID: 31179640). SEMA7A has 2 isoforms with molecular weights of 73 and 75 kDa, respectively.

Protocols

Product Specific Protocols
WB protocol for SEMA7A antibody 67397-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
mouseWB

Cell Commun Signal

Semaphorin 7A interacts with nuclear factor NF-kappa-B p105 via integrin β1 and mediates inflammation

Authors - Xuan Li
View Article