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Conjugate
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Conjugate
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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

SEMA7A Monoclonal antibody, PBS Only

SEMA7A Monoclonal Antibody for WB, Indirect ELISA

Host / Isotype

Mouse / IgG2b

Reactivity

Human

Applications

WB, Indirect ELISA

Conjugate

Unconjugated

CloneNo.

3A5G1

Cat no : 67397-1-PBS

Synonyms

CD108, CDw108, H SEMA K1, H Sema L, JMH, JMH blood group antigen, Sema K1, Sema L, SEMA7A, SEMAK1, SEMAL, Semaphorin 7A, Semaphorin K1, Semaphorin L

Validation Data Gallery

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  • Western Blot (WB) analysis of human red blood cells using SEMA7A Monoclonal antibody (67397-1-Ig)

    WB analysis of human red blood cells using 67397-1-Ig (same clone as 67397-1-PBS)

    human red blood cells were subjected to SDS PAGE followed by western blot with 67397-1-Ig (SEMA7A antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67397-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of human placenta tissue using SEMA7A Monoclonal antibody (67397-1-Ig)

    WB analysis of human placenta using 67397-1-Ig (same clone as 67397-1-PBS)

    human placenta tissue were subjected to SDS PAGE followed by western blot with 67397-1-Ig (SEMA7A antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67397-1-PBS in a different storage buffer formulation.

Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

67397-1-PBS targets SEMA7A in WB, Indirect ELISA applications and shows reactivity with Human samples.

Tested Reactivity Human
Host / Isotype Mouse / IgG2b
Class Monoclonal
Type Antibody
Immunogen SEMA7A fusion protein Ag28804 相同性解析による交差性が予測される生物種
Full Name semaphorin 7A, GPI membrane anchor (John Milton Hagen blood group)
Calculated molecular weight 666 aa, 75 kDa
Observed molecular weight 75-80 kDa
GenBank accession numberBC101643
Gene symbol SEMA7A
Gene ID (NCBI) 8482
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.

Background Information

SEMA7A, also named CD108 and SEMAL, belongs to the semaphorin family. SEMA7A is the only membrane‐associated glycosylphosphatidylinositol (GPI)‐linked semaphorin and plays a role in the regulation of neuronal axon outgrowth. In addition to its role in guiding axon pathfinding during neuronal development, Sema7A has diverse functions in morphogenesis and immune cell control and regulates T cell responses via the α1β1 integrin receptor (PMID: 31179640). SEMA7A has 2 isoforms with molecular weights of 73 and 75 kDa, respectively.