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Species Reactivity
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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

SENP1 Recombinant antibody, PBS Only

SENP1 Recombinant Antibody for WB, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, ELISA

Conjugate

Unconjugated

CloneNo.

240478D3

Cat no : 83594-4-PBS

Synonyms

SuPr2, SuPr 2, Sentrin specific protease 1, SENP 1, 240478D3

Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using SENP1 Recombinant antibody (83594-4-RR)

    WB analysis using 83594-4-RR (same clone as 83594-4-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 83594-4-RR (SENP1 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 83594-4-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human ovarian cancer using SENP1 Recombinant antibody (83594-4-RR)

    IHC staining of human ovarian cancer using 83594-4-RR (same clone as 83594-4-PBS)

    Immunohistochemical analysis of paraffin-embedded human ovarian cancer slide using 83594-4-RR (SENP1 antibody) at dilution of 1:800 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 83594-4-PBS in a different storage buffer formulation.

  • Affinity and Kinetic Characterization of 83594-4-RR

    Affinity and Kinetic Characterization of 83594-4-RR

    Biolayer interferometry (BLl) kinetic assays of 83594-4-RR against Human SENP1 were performed. The affinity constant is below 1 pM.

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Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

The immunogen of 83594-4-PBS is SENP1 Fusion Protein expressed in E. coli.

Tested Reactivity human, mouse, rat
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Immunogen SENP1 fusion protein Ag18028 相同性解析による交差性が予測される生物種
Full Name SUMO1/sentrin specific peptidase 1
Calculated molecular weight 644 aa, 73 kDa
Observed molecular weight73 kDa
GenBank accession numberBC045639
Gene symbol SENP1
Gene ID (NCBI) 29843
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purfication
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.

Background Information

SENP1, Sentrin-specific protease 1, is a cysteine protease that specifically targets members of the small ubiquitin-like modifier (SUMO) protein family. SENP1 catalyzes two functions in the SUMO pathway (PMID: 29506078).The first is the hydrolysis of an alpha-linked peptide bond at the C-terminal end of the small ubiquitin-like modifier (SUMO) propeptides. The second is the deconjugation of SUMO1, SUMO2 and SUMO3 from targeted proteins (PMID: 16253240). SENP1 is located in the nucleus and cytosol.