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Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using SENP1 Recombinant antibody (83594-4-RR)

    WB analysis using 83594-4-RR

    Various lysates were subjected to SDS PAGE followed by western blot with 83594-4-RR (SENP1 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

  • Immunohistochemistry (IHC) staining of human ovarian cancer using SENP1 Recombinant antibody (83594-4-RR)

    IHC staining of human ovarian cancer using 83594-4-RR

    Immunohistochemical analysis of paraffin-embedded human ovarian cancer slide using 83594-4-RR (SENP1 antibody) at dilution of 1:800 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Affinity and Kinetic Characterization of 83594-4-RR

    Affinity and Kinetic Characterization of 83594-4-RR

    Biolayer interferometry (BLl) kinetic assays of 83594-4-RR against Human SENP1 were performed. The affinity constant is below 1 pM.

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SENP1 Recombinant antibody

SENP1 Uni-rAbTM Recombinant Antibody for WB, IHC, ELISA
Cat No. 83594-4-RR
Clone No.240478D3

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, ELISA

Synonyms

SuPr2, SuPr 2, Sentrin specific protease 1, SENP 1, 240478D3

Formulation:  PBS and Azide
PBS and Azide
PBS Only
Conjugate:  Unconjugated
Unconjugated
Size/Concentration: 

-/ -

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国内在庫・納期について

約2万点のプロテインテック製品をコスモバイオ社物流センター（国内）に在庫しています。国内在庫の有無はコスモバイオ社ホームページの「品番検索」でカタログ番号を検索して確認できます。

保証・サポートについて

テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Positive WB detected inHEK-293 cells, HL-60 cells, Jurkat cells, rat testis tiuuse
Positive IHC detected inhuman ovarian cancer
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:5000-1:50000
Immunohistochemistry (IHC)IHC : 1:400-1:1600
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

83594-4-RR targets SENP1 in WB, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Immunogen SENP1 fusion protein Ag18028 相同性解析による交差性が予測される生物種
Full Name SUMO1/sentrin specific peptidase 1
Calculated molecular weight 644 aa, 73 kDa
Observed molecular weight73 kDa
GenBank accession numberBC045639
Gene Symbol SENP1
Gene ID (NCBI) 29843
RRIDAB_3671210
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purfication
UNIPROT IDQ9P0U3
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

SENP1, Sentrin-specific protease 1, is a cysteine protease that specifically targets members of the small ubiquitin-like modifier (SUMO) protein family. SENP1 catalyzes two functions in the SUMO pathway (PMID: 29506078).The first is the hydrolysis of an alpha-linked peptide bond at the C-terminal end of the small ubiquitin-like modifier (SUMO) propeptides. The second is the deconjugation of SUMO1, SUMO2 and SUMO3 from targeted proteins (PMID: 16253240). SENP1 is located in the nucleus and cytosol.

Protocols

Product Specific Protocols
WB protocol for SENP1 antibody 83594-4-RRDownload protocol
IHC protocol for SENP1 antibody 83594-4-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols