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KD/KO Validated

Septin 2 Monoclonal antibody

Name: Septin 2 Monoclonal antibody
Brand: Proteintech
SKU: 60075-1-Ig
Rating: 4.50 (2 reviews)

Septin 2 Monoclonal Antibody for WB, IHC, IF/ICC, FC (Intra), ELISA

Host / Isotype

Mouse / IgG1

Reactivity

human, mouse, rat

Applications

WB, IHC, IF/ICC, FC (Intra), CoIP, ELISA

Conjugate

Unconjugated

Publications

CloneNo.

2F3B2

Reviews (2)

Cat no : 60075-1-Ig

Synonyms

NEDD-5, NEDD5, NEDD 5, KIAA0158, hNedd5

Validation Data Gallery

Filter:

AllWBIHCIF/ICCFC (Intra)

WB analysis using 60075-1-Ig
Various lysates were subjected to SDS PAGE followed by western blot with 60075-1-Ig (Septin 2 antibody) at dilution of 1:100000 incubated at room temperature for 1.5 hours. The membrane was stripped and reblotted with HRP-conjugated Alpha Tubulin Monoclonal antibody (HRP-66031) as loading control.
WB analysis of HEK-293 using 60075-1-Ig
HEK-293 cells were subjected to SDS PAGE followed by western blot with 60075-1-Ig (Septin 2 Antibody) at various dilutions incubated at room temperature for 1.5 hours.
WB analysis of HEK-293 using 60075-1-Ig
WB result of Septin 2 antibody (60075-1-Ig; 1:30000; incubated at room temperature for 1.5 hours) with sh-Control and sh-Septin 2 transfected HEK-293 cells.
WB analysis using 60075-1-Ig
Various lysates were subjected to SDS PAGE followed by western blot with 60075-1-Ig (Septin 2antibody) at dilution of 1:100000 incubated at room temperature for 1.5 hours.
WB analysis of Jurkat using 60075-1-Ig
Jurkat cells were subjected to SDS PAGE followed by western blot with 60075-1-Ig (Septin 2 Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.
WB analysis of fetal human brain using 60075-1-Ig
fetal human brain tissue were subjected to SDS PAGE followed by western blot with 60075-1-Ig (Septin 2 Antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.
WB analysis of HeLa using 60075-1-Ig
HeLa cells were subjected to SDS PAGE followed by western blot with 60075-1-Ig (Septin 2 Antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.
WB analysis of human testis using 60075-1-Ig
human testis tissue were subjected to SDS PAGE followed by western blot with 60075-1-Ig (Septin 2 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.
WB analysis of human brain using 60075-1-Ig
human brain tissue were subjected to SDS PAGE followed by western blot with 60075-1-Ig (Septin 2 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.
IHC staining of human renal cell carcinoma using 60075-1-Ig
Immunohistochemical analysis of paraffin-embedded human renal cell carcinoma tissue slide using 60075-1-Ig (Septin 2 Antibody) at dilution of 1:800 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of human renal cell carcinoma using 60075-1-Ig
Immunohistochemical analysis of paraffin-embedded human renal cell carcinoma tissue slide using 60075-1-Ig (Septin 2 Antibody) at dilution of 1:800 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of human liver cancer using 60075-1-Ig
Immunohistochemical analysis of paraffin-embedded human liver cancer using 60075-1-Ig(Septin 2 antibody) at dilution of 1:50 (under 10x lens).
IHC staining of human liver cancer using 60075-1-Ig
Immunohistochemical analysis of paraffin-embedded human liver cancer using 60075-1-Ig(Septin 2 antibody) at dilution of 1:50 (under 40x lens).
IHC staining of human brain using 60075-1-Ig
Immunohistochemical analysis of paraffin-embedded human brain using 60075-1-Ig(Septin 2 antibody) at dilution of 1:50 (under 10x lens).
IHC staining of human brain using 60075-1-Ig
Immunohistochemical analysis of paraffin-embedded human brain using 60075-1-Ig(Septin 2 antibody) at dilution of 1:50 (under 40x lens).
IF Staining of hTERT-RPE cells using 60075-1-Ig
IF result of anti-Septin 2 (60075-1-Ig, 1:1000) with hTERT-RPE cell by Dr. Moshe Kim. Green: Septin 2. Blue: DNA. 1% PFA fixed.
IF Staining of K-562 using 60075-1-Ig
Immunofluorescent analysis of (4% PFA) fixed K-562 cells using Septin 2 antibody (60075-1-Ig, Clone: 2F3B2 ) at dilution of 1:800 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L).
IF Staining of K-562 using 60075-1-Ig
Immunofluorescent analysis of (4% PFA) fixed K-562 cells using Septin 2 antibody (60075-1-Ig, Clone: 2F3B2 ) at dilution of 1:800 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L).
FC experiment of K-562 using 60075-1-Ig
1X10^6 K-562 cells were intracellularly stained with 0.4 ug Anti-Human Septin 2 (60075-1-Ig, Clone:2F3B2) and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L) at dilution 1:1000 (red), or 0.4 ug Control Antibody. Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

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Cat No. 60075-1-Ig

Size/Concentration

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保証・サポートについて

テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Positive WB detected in	LNCaP cells, Jurkat cells, fetal human brain tissue, human testis tissue, HeLa cells, HEK-293 cells, HepG2 cells, K-562 cells, HSC-T6 cells, NIH/3T3 cells, 4T1 cells, MCF-7 cells, HSCT6 cells, ROS1728 cells, Neuro-2A cells
Positive IHC detected in	human renal cell carcinoma tissue, human brain tissue, human liver cancer tissue **Note: suggested antigen retrieval with TE buffer pH 9.0; () Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0***
Positive IF/ICC detected in	hTERT-RPE cells, K-562 cells
Positive FC (Intra) detected in	K-562 cells

Recommended dilution

Application	Dilution
Western Blot (WB)	WB : 1:20000-1:100000
Immunohistochemistry (IHC)	IHC : 1:50-1:2000
Immunofluorescence (IF)/ICC	IF/ICC : 1:20-1:200
Flow Cytometry (FC) (INTRA)	FC (INTRA) : 0.40 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Published Applications

KD/KO	See 3 publications below
WB	See 12 publications below
IF	See 7 publications below
CoIP	See 1 publications below

Product Information

60075-1-Ig targets Septin 2 in WB, IHC, IF/ICC, FC (Intra), CoIP, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity	human, mouse, rat
Cited Reactivity	human, mouse
Host / Isotype	Mouse / IgG1
Class	Monoclonal
Type	Antibody
Immunogen	Septin 2 fusion protein Ag1946 相同性解析による交差性が予測される生物種
Full Name	septin 2
Calculated molecular weight	41 kDa
Observed molecular weight	42-45 kDa
GenBank accession number	BC014455
Gene symbol	Septin 2
Gene ID (NCBI)	4735
RRID	AB_2187016
Conjugate	Unconjugated
Form	Liquid
Purification Method	Protein G purification
Storage Buffer	PBS with 0.1% sodium azide and 50% glycerol pH 7.3.
Storage Conditions	Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage.

Background Information

Septin 2, also named as DIFF6, KIAA0158, NEDD5, belongs to the septin family. It is a filament-forming cytoskeletal GTPase. Septin 2 is required for normal organization of the actin cytoskeleton. It plays a role in ciliogenesis and collective cell movements. Septin 2 is coordinated expression with Septin 6 and Septin 7. (PMID:21737677)

Protocols

Product Specific Protocols
WB protocol for Septin 2 antibody 60075-1-Ig	Download protocol
IHC protocol for Septin 2 antibody 60075-1-Ig	Download protocol
IF protocol for Septin 2 antibody 60075-1-Ig	Download protocol

Standard Protocols
Click here to view our Standard Protocols

Publications

Species	Application	Title
mouse	IF	Nat Genet A transition zone complex regulates mammalian ciliogenesis and ciliary membrane composition. Authors - Garcia-Gonzalo Francesc R FR View Article
human	WB	Cell Res Regulators of tubulin polyglutamylation control nuclear shape and cilium disassembly by balancing microtubule and actin assembly. Authors - Lei Wang View Article
human	WB	J Cell Biol Septins suppress the release of vaccinia virus from infected cells. Authors - Julia Pfanzelter View Article
mouse	WB,IF	Cell Rep Cdc42EP3/BORG2 and Septin Network Enables Mechano-transduction and the Emergence of Cancer-Associated Fibroblasts. Authors - Fernando Calvo View Article
human	CoIP	iScience Contribution of septins to human platelet structure and function. Authors - Oleg V Kim View Article
human	WB,IF	Cytoskeleton (Hoboken) A requirement for septins and the autophagy receptor p62 in the proliferation of intracellular Shigella. Authors - Damián Lobato-Márquez KD Validated View Article

Reviews

Add a review

The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.

Amaury (Verified Customer) (03-29-2022)

I performed immunofluorescence on K562. It gave me very nice immunolabelling. I used ice-cold methanol to permeabilize the cells.

Applications: Immunofluorescence
Cell Tissue Type: K562

Septin 2 Antibody Immunofluorescence validation ( dilution) in K562 (Cat no:60075-1-Ig)

Stephen (Verified Customer) (01-31-2019)

works fine for WB in HeLa cells 10ug of protein lysate loaded onto 10% NuPAGE gel run I in 1XMOPS buffer blot transferred onto nitrocelluloseAntibody used at 1:2000 incubated overnight at 4 degrees t in 5% milk PBST solution.

Applications: Western Blot,
Primary Antibody Dilution: 1:2000
Cell Tissue Type: HeLa

Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

Septin 2 Monoclonal antibody

Septin 2 Monoclonal Antibody for WB, IHC, IF/ICC, FC (Intra), ELISA

Validation Data Gallery

WB analysis using 60075-1-Ig

WB analysis of HEK-293 using 60075-1-Ig

WB analysis of HEK-293 using 60075-1-Ig

WB analysis using 60075-1-Ig

WB analysis of Jurkat using 60075-1-Ig

WB analysis of fetal human brain using 60075-1-Ig

WB analysis of HeLa using 60075-1-Ig

WB analysis of human testis using 60075-1-Ig

WB analysis of human brain using 60075-1-Ig

IHC staining of human renal cell carcinoma using 60075-1-Ig

IHC staining of human renal cell carcinoma using 60075-1-Ig

IHC staining of human liver cancer using 60075-1-Ig

IHC staining of human liver cancer using 60075-1-Ig

IHC staining of human brain using 60075-1-Ig

IHC staining of human brain using 60075-1-Ig

IF Staining of hTERT-RPE cells using 60075-1-Ig

IF Staining of K-562 using 60075-1-Ig

IF Staining of K-562 using 60075-1-Ig

FC experiment of K-562 using 60075-1-Ig

Tested Applications

Recommended dilution

Published Applications

Product Information

Background Information

Protocols

Publications

A transition zone complex regulates mammalian ciliogenesis and ciliary membrane composition.

Regulators of tubulin polyglutamylation control nuclear shape and cilium disassembly by balancing microtubule and actin assembly.

Septins suppress the release of vaccinia virus from infected cells.

Cdc42EP3/BORG2 and Septin Network Enables Mechano-transduction and the Emergence of Cancer-Associated Fibroblasts.

Contribution of septins to human platelet structure and function.

A requirement for septins and the autophagy receptor p62 in the proliferation of intracellular Shigella.

Reviews