Various lysates were subjected to SDS PAGE followed by western blot with 60075-1-Ig (Septin 2 antibody) at dilution of 1:100000 incubated at room temperature for 1.5 hours. The membrane was stripped and reblotted with HRP-conjugated Alpha Tubulin Monoclonal antibody (HRP-66031) as loading control.

HEK-293 cells were subjected to SDS PAGE followed by western blot with 60075-1-Ig (Septin 2 Antibody) at various dilutions incubated at room temperature for 1.5 hours.

WB result of Septin 2 antibody (60075-1-Ig; 1:30000; incubated at room temperature for 1.5 hours) with sh-Control and sh-Septin 2 transfected HEK-293 cells.

fetal human brain tissue were subjected to SDS PAGE followed by western blot with 60075-1-Ig (Septin 2 Antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

HeLa cells were subjected to SDS PAGE followed by western blot with 60075-1-Ig (Septin 2 Antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

human brain tissue were subjected to SDS PAGE followed by western blot with 60075-1-Ig (Septin 2 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

human testis tissue were subjected to SDS PAGE followed by western blot with 60075-1-Ig (Septin 2 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

Various lysates were subjected to SDS PAGE followed by western blot with 60075-1-Ig (Septin 2antibody) at dilution of 1:100000 incubated at room temperature for 1.5 hours.

Jurkat cells were subjected to SDS PAGE followed by western blot with 60075-1-Ig (Septin 2 Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

Immunohistochemical analysis of paraffin-embedded human renal cell carcinoma tissue slide using 60075-1-Ig (Septin 2 Antibody) at dilution of 1:800 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

Immunohistochemical analysis of paraffin-embedded human renal cell carcinoma tissue slide using 60075-1-Ig (Septin 2 Antibody) at dilution of 1:800 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

Immunohistochemical analysis of paraffin-embedded human liver cancer using 60075-1-Ig(Septin 2 antibody) at dilution of 1:50 (under 10x lens).

Immunohistochemical analysis of paraffin-embedded human liver cancer using 60075-1-Ig(Septin 2 antibody) at dilution of 1:50 (under 40x lens).

Immunohistochemical analysis of paraffin-embedded human brain using 60075-1-Ig(Septin 2 antibody) at dilution of 1:50 (under 10x lens).

Immunohistochemical analysis of paraffin-embedded human brain using 60075-1-Ig(Septin 2 antibody) at dilution of 1:50 (under 40x lens).

IF result of anti-Septin 2 (60075-1-Ig, 1:1000) with hTERT-RPE cell by Dr. Moshe Kim. Green: Septin 2. Blue: DNA. 1% PFA fixed.

Immunofluorescent analysis of (4% PFA) fixed K-562 cells using Septin 2 antibody (60075-1-Ig, Clone: 2F3B2 ) at dilution of 1:800 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L).

Immunofluorescent analysis of (4% PFA) fixed K-562 cells using Septin 2 antibody (60075-1-Ig, Clone: 2F3B2 ) at dilution of 1:800 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L) (SA00013-1).

1X10^6 K-562 cells were intracellularly stained with 0.4 ug Anti-Human Septin 2 (60075-1-Ig, Clone:2F3B2) and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L) at dilution 1:1000 (red), or 0.4 ug Control Antibody. Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).