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Validation Data Gallery

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  • Immunofluorescence (IF) / fluorescent staining of HepG2 cells using CoraLite®594-conjugated Septin 2 Monoclonal antibo (CL594-60075)

    IF Staining of HepG2 using CL594-60075

    Immunofluorescent analysis of (-20°C Ethanol) fixed HepG2 cells using CoraLite®594 Septin 2 antibody (CL594-60075, Clone: 2F3B2 ) at dilution of 1:200.

  • Immunofluorescence (IF) / fluorescent staining of MCF-7 cells using CoraLite®594-conjugated Septin 2 Monoclonal antibo (CL594-60075)

    IF Staining of MCF-7 using CL594-60075

    Immunofluorescent analysis of (-20°C Methanol) fixed MCF-7 cells using CoraLite®594 Septin 2 antibody (CL594-60075, Clone: 2F3B2 ) at dilution of 1:200.

  • Immunofluorescence (IF) / fluorescent staining of K-562 cells using CoraLite®594-conjugated Septin 2 Monoclonal antibo (CL594-60075)

    IF Staining of K-562 using CL594-60075

    Immunofluorescent analysis of (4% PFA) fixed K-562 cells using CoraLite®594 Septin 2 antibody (CL594-60075, Clone: 2F3B2 ) at dilution of 1:200.

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  • Featured Product
  • KD/KO Validated

CoraLite®594-conjugated Septin 2 Monoclonal antibody

Septin 2 Monoclonal Antibody for IF/ICC
Cat No. CL594-60075
Clone No.2F3B2

Host / Isotype

Mouse / IgG1

Reactivity

human, mouse, rat

Applications

IF/ICC

Synonyms

NEDD-5, NEDD5, NEDD 5, KIAA0158, hNedd5

Formulation:  PBS and Azide
PBS and Azide
Conjugate:  CoraLite®594
Size/Concentration: 

-/ -

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保証・サポートについて

テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Positive IF/ICC detected inMCF-7 cells, HepG2 cells, K-562 cells

Recommended dilution

ApplicationDilution
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

CL594-60075 targets Septin 2 in IF/ICC applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Immunogen Septin 2 fusion protein Ag1946 相同性解析による交差性が予測される生物種
Full Name septin 2
Calculated molecular weight 41 kDa
Observed molecular weight 42-45 kDa
GenBank accession numberBC014455
Gene Symbol Septin 2
Gene ID (NCBI) 4735
RRIDAB_2883416
Conjugate CoraLite®594 Fluorescent Dye
Excitation/Emission maxima wavelengths588 nm / 604 nm
Form Liquid
Purification MethodProtein G purification
UNIPROT IDQ15019
Storage Buffer PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
Storage ConditionsStore at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

Septin 2, also named as DIFF6, KIAA0158, NEDD5, belongs to the septin family. It is a filament-forming cytoskeletal GTPase. Septin 2 is required for normal organization of the actin cytoskeleton. It plays a role in ciliogenesis and collective cell movements. Septin 2 is coordinated expression with Septin 6 and Septin 7. (PMID:21737677)

Protocols

Product Specific Protocols
IF protocol for CL594 Septin 2 antibody CL594-60075Download protocol
Standard Protocols
Click here to view our Standard Protocols