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Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using SEPT9 Recombinant antibody (82844-2-RR)

    WB analysis using 82844-2-RR (same clone as 82844-2-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 82844-2-RR (SEPT9 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 82844-2-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of A549 cells using SEPT9 Recombinant antibody (82844-2-RR)

    WB analysis of A549 using 82844-2-RR (same clone as 82844-2-PBS)

    WB result of SEPT9 antibody (82844-2-RR; 1:2000; incubated at room temperature for 1.5 hours) with sh-Control and sh-SEPT9 transfected A549 cells. This data was developed using the same antibody clone with 82844-2-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of various lysates using SEPT9 Recombinant antibody (82844-2-RR)

    WB analysis using 82844-2-RR (same clone as 82844-2-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 82844-2-RR (SEPT9 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 82844-2-PBS in a different storage buffer formulation.

  • Immunoprecipitation (IP) experiment of mouse brain tissue using SEPT9 Recombinant antibody (82844-2-RR)

    IP experiment of mouse brain using 82844-2-RR (same clone as 82844-2-PBS)

    IP result of anti-SEPT9 (IP:82844-2-RR, 4ug; Detection:82844-2-RR 1:1000) with mouse brain tissue lysate 1680 ug. This data was developed using the same antibody clone with 82844-2-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human colon tissue using SEPT9 Recombinant antibody (82844-2-RR)

    IHC staining of human colon using 82844-2-RR (same clone as 82844-2-PBS)

    Immunohistochemical analysis of paraffin-embedded human colon tissue slide using 82844-2-RR (SEPT9 antibody) at dilution of 1:1000 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 82844-2-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human colon cancer using SEPT9 Recombinant antibody (82844-2-RR)

    IHC staining of human colon cancer using 82844-2-RR (same clone as 82844-2-PBS)

    Immunohistochemical analysis of paraffin-embedded human colon cancer slide using 82844-2-RR (SEPT9 antibody) at dilution of 1:400 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 82844-2-PBS in a different storage buffer formulation.

  • Affinity and Kinetic Characterization of 82844-2-RR

    Affinity and Kinetic Characterization of 82844-2-RR

    Biolayer interferometry (BLl) kinetic assays of 82844-2-RR against Human SEPT9 were performed. The affinity constant is below 1 pM.

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  • Featured Product
  • KD/KO Validated

SEPT9 Recombinant antibody, PBS Only

SEPT9 Uni-rAbTM Recombinant Antibody for WB, IHC, IP, Indirect ELISA
Cat No. 82844-2-PBS
Clone No.2E7

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IHC, IP, Indirect ELISA

Synonyms

septin 9, MSF, MLL septin-like fusion protein MSF-A, MLL septin-like fusion protein, KIAA0991

Formulation:  PBS Only
PBS and Azide
PBS Only
Conjugate:  Unconjugated
Size/Concentration: 

-/ -

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Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

82844-2-PBS targets SEPT9 in WB, IHC, IP, Indirect ELISA applications and shows reactivity with human, mouse samples.

Tested Reactivity human, mouse
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Immunogen SEPT9 fusion protein Ag1218 相同性解析による交差性が予測される生物種
Full Name septin 9
Calculated molecular weight 64 kDa
Observed molecular weight 65 kDa, 38-40 kDa
GenBank accession numberBC021192
Gene Symbol SEPT9
Gene ID (NCBI) 10801
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
UNIPROT IDQ9UHD8
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.

Background Information

SEPT9 belongs to the septin family which is a group of highly conserved GTP-binding proteins in eukaryotes. SEPT9 plays a role in cytokinesis, the final stage of cell division. SEPT9 gene methylation has been implicated as a biomarker for colorectal cancer(CRC).