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SEPT9 Recombinant antibody

SEPT9 Recombinant Antibody for WB, IP, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IP, ELISA

Conjugate

Unconjugated

CloneNo.

2E7

Cat no : 82844-2-RR

Synonyms

septin 9, MSF, MLL septin-like fusion protein MSF-A, MLL septin-like fusion protein, KIAA0991

Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using SEPT9 Recombinant antibody (82844-2-RR)

    WB analysis using 82844-2-RR

    Various lysates were subjected to SDS PAGE followed by western blot with 82844-2-RR (SEPT9 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of A549 cells using SEPT9 Recombinant antibody (82844-2-RR)

    WB analysis of A549 using 82844-2-RR

    WB result of SEPT9 antibody (82844-2-RR; 1:2000; incubated at room temperature for 1.5 hours) with sh-Control and sh-SEPT9 transfected A549 cells.

  • Western Blot (WB) analysis of various lysates using SEPT9 Recombinant antibody (82844-2-RR)

    WB analysis using 82844-2-RR

    Various lysates were subjected to SDS PAGE followed by western blot with 82844-2-RR (SEPT9 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

  • Immunoprecipitation (IP) experiment of mouse brain tissue using SEPT9 Recombinant antibody (82844-2-RR)

    IP experiment of mouse brain using 82844-2-RR

    IP result of anti-SEPT9 (IP:82844-2-RR, 4ug; Detection:82844-2-RR 1:1000) with mouse brain tissue lysate 1680 ug.

  • Affinity and Kinetic Characterization of 82844-2-RR

    Affinity and Kinetic Characterization of 82844-2-RR

    Biolayer interferometry (BLl) kinetic assays of 82844-2-RR against Human SEPT9 were performed. The affinity constant is below 1 pM.

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Tested Applications

Positive WB detected inA549 cells, HepG2 cells, mouse brain tissue, mouse spleen tissue
Positive IP detected inmouse brain tissue

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:2000-1:10000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

82844-2-RR targets SEPT9 in WB, IP, ELISA applications and shows reactivity with human, mouse samples.

Tested Reactivity human, mouse
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Immunogen SEPT9 fusion protein Ag1218 相同性解析による交差性が予測される生物種
Full Name septin 9
Calculated molecular weight 64 kDa
Observed molecular weight 65 kDa, 38-40 kDa
GenBank accession numberBC021192
Gene symbol SEPT9
Gene ID (NCBI) 10801
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

SEPT9 belongs to the septin family which is a group of highly conserved GTP-binding proteins in eukaryotes. SEPT9 plays a role in cytokinesis, the final stage of cell division. SEPT9 gene methylation has been implicated as a biomarker for colorectal cancer(CRC).

Protocols

Product Specific Protocols
WB protocol for SEPT9 antibody 82844-2-RRDownload protocol
IP protocol for SEPT9 antibody 82844-2-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols