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Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using SEPT9 Recombinant antibody (82844-2-RR)

    WB analysis using 82844-2-RR

    Various lysates were subjected to SDS PAGE followed by western blot with 82844-2-RR (SEPT9 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of A549 cells using SEPT9 Recombinant antibody (82844-2-RR)

    WB analysis of A549 using 82844-2-RR

    WB result of SEPT9 antibody (82844-2-RR; 1:2000; incubated at room temperature for 1.5 hours) with sh-Control and sh-SEPT9 transfected A549 cells.

  • Western Blot (WB) analysis of various lysates using SEPT9 Recombinant antibody (82844-2-RR)

    WB analysis using 82844-2-RR

    Various lysates were subjected to SDS PAGE followed by western blot with 82844-2-RR (SEPT9 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

  • Immunoprecipitation (IP) experiment of mouse brain tissue using SEPT9 Recombinant antibody (82844-2-RR)

    IP experiment of mouse brain using 82844-2-RR

    IP result of anti-SEPT9 (IP:82844-2-RR, 4ug; Detection:82844-2-RR 1:1000) with mouse brain tissue lysate 1680 ug.

  • Immunohistochemistry (IHC) staining of human colon cancer using SEPT9 Recombinant antibody (82844-2-RR)

    IHC staining of human colon cancer using 82844-2-RR

    Immunohistochemical analysis of paraffin-embedded human colon cancer slide using 82844-2-RR (SEPT9 antibody) at dilution of 1:400 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human colon tissue using SEPT9 Recombinant antibody (82844-2-RR)

    IHC staining of human colon using 82844-2-RR

    Immunohistochemical analysis of paraffin-embedded human colon tissue slide using 82844-2-RR (SEPT9 antibody) at dilution of 1:1000 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Affinity and Kinetic Characterization of 82844-2-RR

    Affinity and Kinetic Characterization of 82844-2-RR

    Biolayer interferometry (BLl) kinetic assays of 82844-2-RR against Human SEPT9 were performed. The affinity constant is below 1 pM.

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  • Featured Product
  • KD/KO Validated

SEPT9 Recombinant antibody

SEPT9 Recombinant Antibody for WB, IHC, IP, ELISA
Cat No. 82844-2-RR
Clone No.2E7

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IHC, IP, ELISA

Synonyms

septin 9, MSF, MLL septin-like fusion protein MSF-A, MLL septin-like fusion protein, KIAA0991

Formulation:  PBS and Azide
PBS and Azide
PBS Only
Conjugate:  Unconjugated
Unconjugated
Size/Concentration: 

-/ -

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保証・サポートについて

テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Positive WB detected inA549 cells, HepG2 cells, mouse brain tissue, mouse spleen tissue
Positive IP detected inmouse brain tissue
Positive IHC detected inhuman colon tissue, human colon cancer
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:2000-1:10000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)IHC : 1:500-1:2000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

82844-2-RR targets SEPT9 in WB, IHC, IP, ELISA applications and shows reactivity with human, mouse samples.

Tested Reactivity human, mouse
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Immunogen SEPT9 fusion protein Ag1218 相同性解析による交差性が予測される生物種
Full Name septin 9
Calculated molecular weight 64 kDa
Observed molecular weight 65 kDa, 38-40 kDa
GenBank accession numberBC021192
Gene Symbol SEPT9
Gene ID (NCBI) 10801
RRIDAB_3670573
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
UNIPROT IDQ9UHD8
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

SEPT9 belongs to the septin family which is a group of highly conserved GTP-binding proteins in eukaryotes. SEPT9 plays a role in cytokinesis, the final stage of cell division. SEPT9 gene methylation has been implicated as a biomarker for colorectal cancer(CRC).

Protocols

Product Specific Protocols
WB protocol for SEPT9 antibody 82844-2-RRDownload protocol
IHC protocol for SEPT9 antibody 82844-2-RRDownload protocol
IP protocol for SEPT9 antibody 82844-2-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols