Serpin C1/Antithrombin III Monoclonal antibody
Serpin C1/Antithrombin III Monoclonal Antibody for WB, IHC, IF/ICC, ELISA
Host / Isotype
Mouse / IgG1
Reactivity
human, mouse, rat
Applications
WB, IHC, IF/ICC, ELISA
Conjugate
Unconjugated
CloneNo.
2B2E5
Cat no : 66052-1-Ig
Synonyms
Validation Data Gallery
Tested Applications
| Positive WB detected in | human plasma, human brain tissue, human testis tissue, L02 cells, rat brain tissue |
| Positive IHC detected in | human liver cancer tissue, human liver tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Positive IF/ICC detected in | HepG2 cells, L02 cells |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:2000-1:10000 |
| Immunohistochemistry (IHC) | IHC : 1:50-1:500 |
| Immunofluorescence (IF)/ICC | IF/ICC : 1:400-1:1600 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Published Applications
| WB | See 1 publications below |
| IF | See 1 publications below |
Product Information
66052-1-Ig targets Serpin C1/Antithrombin III in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human, mouse, rat samples.
| Tested Reactivity | human, mouse, rat |
| Cited Reactivity | human, mouse |
| Host / Isotype | Mouse / IgG1 |
| Class | Monoclonal |
| Type | Antibody |
| Immunogen | Serpin C1/Antithrombin III fusion protein Ag9880 相同性解析による交差性が予測される生物種 |
| Full Name | serpin peptidase inhibitor, clade C (antithrombin), member 1 |
| Calculated molecular weight | 464 aa, 53 kDa |
| Observed molecular weight | 53 kDa |
| GenBank accession number | BC022309 |
| Gene symbol | Serpin C1/Antithrombin III |
| Gene ID (NCBI) | 462 |
| RRID | AB_11042589 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Protein A purification |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
Background Information
SERPINC1, also named Antithrombin III or Antithrombin, is a plasma protease inhibitor and a member of the serpin superfamily. This protein inhibits thrombin as well as other activated serine proteases of the coagulation system, and it regulates the blood coagulation cascade. More than 120 mutations have been identified for this gene, many of which are known to cause antithrombin-III deficiency.
Protocols
| Product Specific Protocols | |
|---|---|
| WB protocol for Serpin C1/Antithrombin III antibody 66052-1-Ig | Download protocol |
| IHC protocol for Serpin C1/Antithrombin III antibody 66052-1-Ig | Download protocol |
| IF protocol for Serpin C1/Antithrombin III antibody 66052-1-Ig | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
| Species | Application | Title |
|---|---|---|
Matrix Biol Proteome-wide and matrisome-specific atlas of the human ovary computes fertility biomarker candidates and open the way for precision oncofertility. | ||
Front Oncol Synergistic effects of nab-PTX and anti-PD-1 antibody combination against lung cancer by regulating the Pi3K/AKT pathway through the Serpinc1 gene |
Reviews
The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.
FH Iram (Verified Customer) (01-15-2021) | Antithrombin III Monoclonal antibody giving a very clear sharp bands
|
FH Susmita (Verified Customer) (09-17-2020) | The antibody works good in mentioned dilution for WB and IHC
 |
