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Validation Data Gallery

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  • Western Blot (WB) analysis of C6 cells using SERPINE2 Polyclonal antibody (11303-1-AP)

    WB analysis of C6 using 11303-1-AP (same clone as 11303-1-PBS)

    C6 cells were subjected to SDS PAGE followed by western blot with 11303-1-AP (SERPINE2 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 11303-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of rat heart tissue using SERPINE2 Polyclonal antibody (11303-1-AP)

    WB analysis of rat heart using 11303-1-AP (same clone as 11303-1-PBS)

    rat heart tissue were subjected to SDS PAGE followed by western blot with 11303-1-AP (SERPINE2 antibody) at dilution of 1:100 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 11303-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of MCF-7 cells using SERPINE2 Polyclonal antibody (11303-1-AP)

    WB analysis of MCF-7 using 11303-1-AP (same clone as 11303-1-PBS)

    MCF7 cells were subjected to SDS PAGE followed by western blot with 11303-1-AP (SERPINE2 antibody) at dilution of 1:100 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 11303-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of A549 cells using SERPINE2 Polyclonal antibody (11303-1-AP)

    WB analysis of A549 using 11303-1-AP (same clone as 11303-1-PBS)

    A549 cells were subjected to SDS PAGE followed by western blot with 11303-1-AP (SERPINE2 antibody) at dilution of 1:100 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 11303-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of rat brain tissue using SERPINE2 Polyclonal antibody (11303-1-AP)

    WB analysis of rat brain using 11303-1-AP (same clone as 11303-1-PBS)

    rat brain tissue were subjected to SDS PAGE followed by western blot with 11303-1-AP (SERPINE2 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 11303-1-PBS in a different storage buffer formulation.

  • Immunoprecipitation (IP) experiment of A549 cells using SERPINE2 Polyclonal antibody (11303-1-AP)

    IP experiment of A549 using 11303-1-AP (same clone as 11303-1-PBS)

    IP result of anti-SERPINE2 (IP:11303-1-AP, 3ug; Detection:11303-1-AP 1:300) with A549 cells lysate 2800ug. This data was developed using the same antibody clone with 11303-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human testis tissue using SERPINE2 Polyclonal antibody (11303-1-AP)

    IHC staining of human testis using 11303-1-AP (same clone as 11303-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human testis slide using 11303-1-AP (SERPINE2 Antibody) at dilution of 1:50. This data was developed using the same antibody clone with 11303-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human testis tissue using SERPINE2 Polyclonal antibody (11303-1-AP)

    IHC staining of human testis using 11303-1-AP (same clone as 11303-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human testis slide using 11303-1-AP (SERPINE2 Antibody) at dilution of 1:50. This data was developed using the same antibody clone with 11303-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human pancreas cancer tissue using SERPINE2 Polyclonal antibody (11303-1-AP)

    IHC staining of human pancreas cancer using 11303-1-AP (same clone as 11303-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human pancreas cancer using 11303-1-AP (SERPINE2 antibody) at dilution of 1:50 (under 10x lens). This data was developed using the same antibody clone with 11303-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of human testis tissue using SERPINE2 Polyclonal antibody (11303-1-AP)

    IF Staining of human testis using 11303-1-AP (same clone as 11303-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed human testis tissue using SERPINE2 antibody (11303-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L). This data was developed using the same antibody clone with 11303-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of human testis tissue using SERPINE2 Polyclonal antibody (11303-1-AP)

    IF Staining of human testis using 11303-1-AP (same clone as 11303-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed human testis tissue using SERPINE2 antibody (11303-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L). This data was developed using the same antibody clone with 11303-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of mouse testis tissue using SERPINE2 Polyclonal antibody (11303-1-AP)

    IF Staining of mouse testis using 11303-1-AP (same clone as 11303-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed mouse testis tissue using SERPINE2 antibody (11303-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L). This data was developed using the same antibody clone with 11303-1-PBS in a different storage buffer formulation.

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  • Featured Product
  • KD/KO Validated

SERPINE2 Polyclonal antibody, PBS Only

SERPINE2 Polyclonal Antibody for WB, IHC, IF-P, IP, Indirect ELISA
Cat No. 11303-1-PBS

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF-P, IP, Indirect ELISA

Synonyms

Formulation:  PBS Only
PBS and Azide
PBS Only
Conjugate:  Unconjugated
Size/Concentration: 

-/ -

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Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

11303-1-PBS targets SERPINE2 in WB, IHC, IF-P, IP, Indirect ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen SERPINE2 fusion protein Ag1830 相同性解析による交差性が予測される生物種
Full Name serpin peptidase inhibitor, clade E (nexin, plasminogen activator inhibitor type 1), member 2
Calculated molecular weight 44 kDa
Observed molecular weight 44-50 kDa
GenBank accession numberBC015663
Gene Symbol SERPINE2
Gene ID (NCBI) 5270
RRIDAB_10732939
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDP07093
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.

Background Information

SERPINE2 also called protease nexin-1 or glial-derived neurite promoting factor, is a member of the serine protease inhibitor (SERPIN) superfamily. It would be a potent inhibitor of the urokinase-plasminogen activator (uPA), tissue-type PA (tPA), thrombin, trypsin, factor Xia, and prostasin. SERPINE2 is widely expressed in various tissues, including endothelial cells, fibroblasts, smooth muscle cells, tumor cells, glial cells, neurons, and placental cells.