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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

SERPINE2 Recombinant antibody, PBS Only

SERPINE2 Recombinant Antibody for Indirect ELISA, WB

Host / Isotype

Rabbit / IgG

Reactivity

rat, mouse, human

Applications

Indirect ELISA, WB

Conjugate

Unconjugated

CloneNo.

5O16

Cat no : 82732-5-PBS

Synonyms

PI7, PN1, Serpin E2, PI 7, Peptidase inhibitor 7

Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using SERPINE2 Recombinant antibody (82732-5-RR)

    WB analysis using 82732-5-RR (same clone as 82732-5-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 82732-5-RR (SERPINE2 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 82732-5-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of various lysates using SERPINE2 Recombinant antibody (82732-5-RR)

    WB analysis using 82732-5-RR (same clone as 82732-5-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 82732-5-RR (SERPINE2 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 82732-5-PBS in a different storage buffer formulation.

  • Flow cytometry (FC) experiment of A549 cells using SERPINE2 Recombinant antibody (82732-5-RR)

    FC experiment of A549 using 82732-5-RR (same clone as 82732-5-PBS)

    1x10^6 A549 cells were intracellularly stained with 0.25 ug SERPINE2 Recombinant antibody (82732-5-RR, Clone:5O16) and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2)(red), or 0.25 ug Rabbit IgG control Rabbit PolyAb (30000-0-AP) (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C). This data was developed using the same antibody clone with 82732-5-PBS in a different storage buffer formulation.

  • Affinity and Kinetic Characterization of 82732-5-RR

    Affinity and Kinetic Characterization of 82732-5-RR

    Biolayer interferometry (BLl) kinetic assays of 82732-5-RR against Human SERPINE2 were performed. The affinity constant is below 1 pM.

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Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

82732-5-PBS targets SERPINE2 in Indirect ELISA, WB applications and shows reactivity with rat, mouse, human samples.

Tested Reactivity rat, mouse, human
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Immunogen SERPINE2 fusion protein Ag1830 相同性解析による交差性が予測される生物種
Full Name serpin peptidase inhibitor, clade E (nexin, plasminogen activator inhibitor type 1), member 2
Calculated molecular weight 44 kDa
Observed molecular weight 44-50 kDa
GenBank accession numberBC015663
Gene symbol SERPINE2
Gene ID (NCBI) 5270
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.

Background Information

SERPINE2 also called protease nexin-1 or glial-derived neurite promoting factor, is a member of the serine protease inhibitor (SERPIN) superfamily. It would be a potent inhibitor of the urokinase-plasminogen activator (uPA), tissue-type PA (tPA), thrombin, trypsin, factor Xia, and prostasin. SERPINE2 is widely expressed in various tissues, including endothelial cells, fibroblasts, smooth muscle cells, tumor cells, glial cells, neurons, and placental cells.